Correlation of the content of hepatitis B core antigen in peripheral blood mononuclear cells with HBV virus load

被引：6
作者：
Peng, Yanzhong [1 ]
Xiong, Xiaojia [1 ]
Tong, Xindeng [1 ]
Li, Min [1 ]
Yang, Xifei [2 ]
Hu, Guoxin [1 ]
Geng, Yijie [2 ]
Li, Jianxin [1 ]
机构：
[1] Peking Univ, Shenzhen Hosp, Shenzhen 518000, Guangdong, Peoples R China
[2] Shenzhen Ctr Dis Control & Prevent, Key Lab Modern Toxicol Shenzhen, 8 Longyuan Rd, Shenzhen 518055, Peoples R China
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2016年 / 85卷 / 02期
关键词：
CLSM; HBcAg; HBV; PBMC; LIVER-TRANSPLANTATION; ENZYME-IMMUNOASSAY; DNA; ASSAY; QUANTITATION; DYSFUNCTION; ANTIBODIES;
D O I：
10.1016/j.diagmicrobio.2015.09.017
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The dysfunction of peripheral blood mononuclear cell (PBMC) plays an important role in hepatitis B virus (HBV) chronic infection and tolerance. This study is aimed to explore the changes of expression and distribution of Hepatitis B virus core antigen (HBcAg) in the PBMCs of patients infected with chronic hepatitis B virus by using confocal laser scanning microscopy (CLSM). The levels of HBcAg in PBMCs were correlated with the HBV load in serum, and the change of HBcAg distribution in different PBMC organelles may represent various HBV infection states. HBcAg was mainly distributed in the nuclei of PBMC in the cases of immune tolerance and no inflammatory activity. Taken together, our data suggest that the measurement of HBcAg and its distribution in PBMCs using CLSM may serve as an alternative approach to monitor HBV load and the immune states of HBV infection with ease of using and improved sensitivity. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：154 / 158
页数：5
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