Spectrophotometric determination of cysteine and N-acetylcysteine in pharmaceutical preparations

被引：56

作者：

Abu Eid, M ^{[1
]}

机构：

[1] An Najah Univ, Dept Chem, Nablus, West Bank, Israel

来源：

MIKROCHIMICA ACTA | 1998年 / 129卷 / 1-2期

关键词：

cysteine; N-acetylcysteine; spectrophotometry; ferrozine; pharmaceuticals;

D O I：

10.1007/BF01246854

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An accurate fast spectrophotometric method for the determination of cysteine and N-acetylcysteine is presented, based on the oxidation of these amino acids by ferric ions in the presence of ferrozine, whereby a violet-coloured complex is formed which absorbs at 562 nm. The method was satisfactory for the determination of cysteine and N-acetylcysteine in samples within the range 0.02-6.00 mu g ml(-1). Effects of time, acidity, ferric ions, ferrozine, sodium perchlorate concentrations and the tolerance limit for other amino acids have been reported. The method was applied to the determination of cysteine in amino acids mixtures and N-acetylcysteine in pharmaceutical preparations.

引用

页码：91 / 95

页数：5

共 13 条

[1]

ALEXIEV AA, 1983, MIKROCHIM ACTA, V2, P369

[2] SPECTROPHOTOMETRIC DETERMINATION OF N-ACETYLCYSTEINE IN DRUG FORMULATIONS WITH O-PHTHALALDEHYDE AND ISOLEUCINE [J].