Influence of a mitochondrial genetic defect on capacitative calcium entry and mitochondrial organization in the osteosarcoma cells

被引：15
作者：
Szczepanowska, J [1 ]
Zablocki, K [1 ]
Duszynski, J [1 ]
机构：
[1] M Nencki Inst Expt Biol, Dept Cellular Biochem, PL-02093 Warsaw, Poland
来源：
FEBS LETTERS | 2004年 / 578卷 / 03期
关键词：
osteosarcoma; mitochondria; cytoskeleton; capacitative calcium entry; NARP;
D O I：
10.1016/j.febslet.2004.11.022
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Effects of T8993G mutation in mitochondrial DNA (mtDNA), associated with neurogenical muscle weakness, ataxia and retinitis pigmentosa (NARP), on the cytoskeleton, mitochondrial network and calcium homeostasis in human osteosarcoma cells were investigated. In 98% NARP and rho(0) (lacking mtDNA) cells, the organization of the mitochondrial network and actin cytoskeleton was disturbed. Capacitative calcium entry (CCE) was practically independent of mitochondrial energy status in osteosarcoma cell lines. The significantly slower Ca2+ influx rates observed in 98% NARP and rho(0), in comparison to parental cells, indicates that proper actin cytoskeletal organization is important for CCE in these cells. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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页码：316 / 322
页数：7
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