Gene Expression Changes in Progression of Cervical Neoplasia Revealed by Microarray Analysis of Cervical Neoplastic Keratinocytes

被引：53

作者：

Rotondo, John Charles ^{[1
]}

Bosi, Silvia ^{[1
]}

Bassi, Cristian ^{[1
]}

Ferracin, Manuela ^{[1
,2
]}

Lanza, Giovanni ^{[1
]}

Gafa, Roberta ^{[1
]}

Magri, Eros ^{[1
]}

Selvatici, Rita ^{[3
]}

Torresani, Stefania ^{[1
]}

Marci, Roberto ^{[1
]}

Garutti, Paola ^{[1
]}

Negrini, Massimo ^{[1
,2
]}

Tognon, Mauro ^{[1
]}

Martini, Fernanda ^{[1
]}

机构：

[1] Univ Ferrara, Dept Morphol Surg & Expt Med, I-44121 Ferrara, Italy

[2] Univ Ferrara, Lab Tecnol Terapie Avanzate, Tecnopolo Univ Ferrara, I-44121 Ferrara, Italy

[3] Univ Ferrara, Dept Med Sci, I-44121 Ferrara, Italy

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2015年 / 230卷 / 04期

关键词：

INTRAEPITHELIAL NEOPLASIA; HUMAN-PAPILLOMAVIRUS; PROMOTER METHYLATION; NEUROBLASTOMA-CELLS; TIMP-3; EXPRESSION; NATURAL-HISTORY; YOUNG-WOMEN; CARCINOGENESIS; PROLIFERATION; CANCER;

D O I：

10.1002/jcp.24808

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To evaluate the gene expression changes involved in neoplastic progression of cervical intraepithelial neoplasia. Using microarray analysis, large-scale gene expression profile was carried out on HPV16-CIN2, HPV16-CIN3, and normal cervical keratinocytes derived from two HPV16-CIN2, two HPV-CIN3 lesions, and two corresponding normal cervical tissues, respectively. Differentially expressed genes were analyzed in normal cervical keratinocytes compared with HPV16-CIN2 keratinocytes and in HPV16-CIN2 keratinocytes compared with HPV16-CIN3 keratinocytes; 37 candidate genes with continuously increasing or decreasing expression during CIN progression were identified. One of these genes, phosphoglycerate dehydrogenase, was chosen for further characterization. Quantitative reverse transcription-polymerase chain reaction and immunohistochemical analysis confirmed that expression of phosphoglycerate dehydrogenase consistently increases during progression of CIN toward cancer. Gene expression changes occurring during CIN progression were investigated using microarray analysis, for the first time, in CIN2 and CIN3 keratinocytes naturally infected with HPV16. Phosphoglycerate dehydrogenase is likely to be associated with tumorigenesis and may be a potential prognostic marker for CIN progression. (C) 2014 Wiley Periodicals, Inc.

引用

页码：806 / 812

页数：7

共 42 条

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