Structural analysis of the yeast SWI/SNF chromatin remodeling complex

被引：137
作者：
Smith, CL
Horowitz-Scherer, R
Flanagan, JF
Woodcock, CL
Peterson, CL [1 ]
机构：
[1] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA
[2] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA
来源：
NATURE STRUCTURAL BIOLOGY | 2003年 / 10卷 / 02期
关键词：
D O I：
10.1038/nsb888
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Elucidating the mechanism of ATP-dependent chromatin remodeling is one of the largest challenges in the field of gene regulation. One of the missing pieces in understanding this process is detailed structural information on the enzymes that catalyze the remodeling reactions. Here we use a combination of subunit radio-iodination and scanning transmission electron microscopy to determine the subunit stoichiometry and native molecular weight of the yeast SWI/SNF complex. We also report a three-dimensional reconstruction of yeast SWI/SNF derived from electron micrographs.
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页码：141 / 145
页数：5
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