Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2

被引：10

作者：

Song, Weidu ^{[1
]}

Zhang, Taiyi ^{[1
]}

Lin, Huichao ^{[1
]}

Yang, Yujing ^{[1
]}

Zhao, Gaozhen ^{[1
]}

Huang, Xiaowen ^{[1
]}

机构：

[1] Qilu Univ Technol, Dept Bioengn, State Key Lab Biobased Mat & Green Papermaking, Shandong Acad Sci, Jinan 250300, Peoples R China

来源：

MICROMACHINES | 2022年 / 13卷 / 04期

基金：

中国国家自然科学基金;

关键词：

SARS-CoV-2; nucleic acid testing; polymerase chain reaction; isothermal amplification; microfluidic; CRISPR-Cas systems; ISOTHERMAL AMPLIFICATION; RAPID DETECTION; HIGH-THROUGHPUT; PCR; DNA; CORONAVIRUS; DIAGNOSTICS; TECHNOLOGY; APTAMERS; BACTERIA;

D O I：

10.3390/mi13040636

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nucleic acid testing (NAT) played a crucial role in containing the spread of SARS-CoV-2 during the epidemic. The gold standard technique, the quantitative real-time polymerase chain reaction (qRT-PCR) technique, is currently used by the government and medical boards to detect SARS-CoV-2. Due to the limitations of this technology, it is not capable of meeting the needs of large-scale rapid detection. To solve this problem, many new techniques for detecting nucleic acids of SARS-CoV-2 have been reported. Therefore, a review that systematically and comprehensively introduces and compares various detection technologies is needed. In this paper, we not only review the traditional NAT but also provide an overview of microfluidic-based NAT technologies and summarize and discuss the characteristics and development prospects of these techniques.

引用

页数：18

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