Insights on Klebsiella pneumoniae Biofilms Assembled on Different Surfaces Using Phenotypic and Genotypic Approaches

被引:21
作者
Bandeira, Maria [1 ,2 ]
Borges, Vitor [3 ]
Gomes, Joao P. [3 ]
Duarte, Aida [4 ]
Jordao, Luisa [1 ]
机构
[1] Inst Nacl Saude Dr Ricardo Jorge, Dept Saude Ambiental, Unidade Invest & Desenvolvimento Lisboa, Ave Padre Cruz, P-1649016 Lisbon, Portugal
[2] Univ Lisbon, Inst Super Tecn, Dept Engn Quim, Ave Rovisco Pais, P-1049001 Lisbon, Portugal
[3] Inst Nacl Saude Dr Ricardo Jorge, Dept Doencas Infeciosas, Nucleo Bioinformat, Ave Padre Cruz, P-1649016 Lisbon, Portugal
[4] Univ Lisbon, Fac Farm, Ave Prof Gama Pinto, P-1649003 Lisbon, Portugal
关键词
biofilms; Klebsiella pneumoniae; electron microscopy; extracellular polymeric substances (EPS); whole genome sequencing (WGS); healthcare associated infections (HAIs); ENTEROBACTERIACEAE; FIMBRIAE; RESISTANCE; DIVERSITY; CELLULOSE; OUTBREAK; ADHESIN; RIBOSE; CURLI;
D O I
10.3390/microorganisms5020016
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Klebsiella pneumoniae is a prominent etiological agent of healthcare associated infections (HAIs). In this context, multidrug-resistant and biofilm-producing bacteria are of special public health concern due to the difficulties associated with treatment of human infections and eradication from hospital environments. Here, in order to study the impact of medical devices-associated materials on the biofilm dynamics, we performed biofilm phenotypic analyses through a classic and a new scanning electron microscopy (SEM) technique for three multidrug-resistant K. pneumoniae isolates growing on polystyrene and silicone. We also applied whole-genome sequencing (WGS) to search for genetic clues underlying biofilm phenotypic differences. We found major differences in the extracellular polymeric substances (EPS) content among the three strains, which were further corroborated by in-depth EPS composition analysis. WGS analysis revealed a high nucleotide similarity within the core-genome, but relevant differences in the accessory genome that may account for the detected biofilm phenotypic dissimilarities, such as genes already associated with biofilm formation in other pathogenic bacteria (e.g., genes coding haemogglutinins and haemolysins). These data reinforce that the research efforts to defeat bacterial biofilms should take into account that their dynamics may be contingent on the medical devices-associated materials.
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页数:16
相关论文
共 43 条
[1]   Biofilm formation and dispersal in Gram-positive bacteria [J].
Abee, Tjakko ;
Kovacs, Akos T. ;
Kuipers, Oscar P. ;
van der Veen, Stijn .
CURRENT OPINION IN BIOTECHNOLOGY, 2011, 22 (02) :172-179
[2]   Biofilm matrix of Candida albicans and Candida tropicalis:: chemical composition and role in drug resistance [J].
Al-Fattani, Mohammed A. ;
Douglas, L. Julia .
JOURNAL OF MEDICAL MICROBIOLOGY, 2006, 55 (08) :999-1008
[3]  
[Anonymous], MICROSC MICROANAL
[4]   The role of filamentous hemagglutinin adhesin in adherence and biofilm formation in Acinetobacter baumannii ATCC19606T [J].
Astaneh, Shakiba Darvish Alipour ;
Rasooli, Iraj ;
Gargari, Seyed Latif Mousavi .
MICROBIAL PATHOGENESIS, 2014, 74 :42-49
[5]   Purification and Characterization of Biofilm-Associated EPS Exopolysaccharides from ESKAPE Organisms and Other Pathogens [J].
Bales, Patrick M. ;
Renke, Emilija Miljkovic ;
May, Sarah L. ;
Shen, Yang ;
Nelson, Daniel C. .
PLOS ONE, 2013, 8 (06)
[6]   Exploring Dangerous Connections between Klebsiella pneumoniae Biofilms and Healthcare-Associated Infections [J].
Bandeira, Maria ;
Carvalho, Patricia Almeida ;
Duarte, Aida ;
Jordao, Luisa .
PATHOGENS, 2014, 3 (03) :720-731
[7]   Genomic Definition of Hypervirulent and Multidrug-Resistant Klebsiella pneumoniae Clonal Groups [J].
Bialek-Davenet, Suzanne ;
Criscuolo, Alexis ;
Ailloud, Florent ;
Passet, Virginie ;
Jones, Louis ;
Delannoy-Vieillard, Anne-Sophie ;
Garin, Benoit ;
Le Hello, Simon ;
Arlet, Guillaume ;
Nicolas-Chanoine, Marie-Helene ;
Decre, Dominique ;
Brisse, Sylvain .
EMERGING INFECTIOUS DISEASES, 2014, 20 (11) :1812-1820
[8]   Medical Biofilms [J].
Bryers, James D. .
BIOTECHNOLOGY AND BIOENGINEERING, 2008, 100 (01) :1-18
[9]  
Calisto F., 2012, Revista Portuguesa de Doencas Infecciosas, V8, P127
[10]   Identification of Protein Domains on Major Pilin MrkA That Affects the Mechanical Properties of Klebsiella pneumoniae Type 3 Fimbriae [J].
Chan, Chia-Han ;
Chen, Feng-Jung ;
Huang, Ying-Jung ;
Chen, Shin-Yu ;
Liu, Kuo-Liang ;
Wang, Zhe-Chong ;
Peng, Hwei-Ling ;
Yew, Tri-Rung ;
Liu, Cheng-Hsien ;
Liou, Gunn-Guang ;
Hsu, Ken Y. ;
Chang, Hwan-You ;
Hsu, Long .
LANGMUIR, 2012, 28 (19) :7428-7435