Isolation and characterization of antimicrobial peptides derived from Bacillus subtilis E20-fermented soybean meal and its use for preventing Vibrio infection in shrimp aquaculture

被引：80
作者：
Cheng, Ann-Chang [1 ]
Lin, Hsueh-Li [2 ]
Shiu, Ya-Li [2 ]
Tyan, Yu-Chang [3 ]
Liu, Chun-Hung [2 ]
机构：
[1] Natl Kaohsiung Marine Univ, Dept & Grad Inst Aquaculture, Kaohsiung 811, Taiwan
[2] Natl Pingtung Univ Sci & Technol, Dept Aquaculture, Pingtung 91201, Taiwan
[3] Kaohsiung Med Univ, Dept Med Imaging & Radiol Sci, Kaohsiung 807, Taiwan
来源：
FISH & SHELLFISH IMMUNOLOGY | 2017年 / 67卷
关键词：
Bacillus subtilis E20; FerMented soybean meal; White shrimp; Antimicrobial peptides; Vibriosis; HALIOTIS-DIVERSICOLOR-SUPERTEXTA; POLYMERASE-CHAIN-REACTION; CULTURED SMALL ABALONE; LITOPENAEUS-VANNAMEI; WHITE SHRIMP; GROWTH-PERFORMANCE; BIOACTIVE PEPTIDES; EDIBLE FILM; FISH-MEAL; FERMENTATION;
D O I：
10.1016/j.fsi.2017.06.006
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Bacillus subtilis E20-fermented soybean meal (FSBM) was found to produce antimicrobial peptides (AMPs) with great antimicrobial activity against Vibrio alginolyticus (VA) and V parahaemolyticus (VP). Three AMPs were purified with a 5 kDa ultrafiltration, Sephadex G-15 column and reverse-phase high-performance liquid chromatography (RP-HPLC). The FSB-AMP, HTSKALLDMLKRLGK, identified by an RP-nano-ultrapure liquid chromatography (UPLC) electrospray ionization (ESI)-tandem mass spectroscopic (MS/MS) analysis exhibited the highest bactericidal activity against VA and VP compared to the others. The antimicrobial activity assessment indicated that FSB-AMP inhibited the growth of VA and VP with minimal inhibitory concentrations of 72.5 and 72.5 mu M. Alterations in the morphology of VA were observed by scanning electronic microscopy, and membrane disruption of VA and VP was confirmed by fluorescent microscopy with propidium iodide staining. The FSB-AMP was then incorporated into the diet of white shrimp, Litopenaeus vannamei, and a protective effect in shrimp against VP infection was recorded as well as for shrimp fed a diet containing 15% fish meal replaced by B. subtilis E20-FSBM. Results demonstrated that B. subtilis E20-FSBM could be a biofunctional ingredient to prevent vibriosis in shrimp aquaculture. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：270 / 279
页数：10
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