Induction of novel Grp75 isoforms by 2-deoxyglucose in human and murine fibroblasts

被引：35
作者：
Merrick, BA
Walker, VR
He, CY
Patterson, RM
Selkirk, JK
机构：
[1] Lab. of Molecular Carcinogenesis, Natl. Inst. of Environ. Hlth. Sci., Research Triangle Park, NC 27709
[2] Lab. of Molecular Carcinogenesis, NIEHS D4-03, Research Triangle Park, NC 27709
来源：
CANCER LETTERS | 1997年 / 119卷 / 02期
关键词：
Grp75; heat shock proteins; 2-deoxyglucose; fibroblasts;
D O I：
10.1016/S0304-3835(97)00270-X
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Grp75 is a stress-inducible mitochondrial chaperone which has a high homology to senescence-related protein. p66(mot) mortalin. In human cells the mortalin gene assigns to the locus of a putative tumor suppressor gene for myeloid malignancies. In order to study expression and localization of Grp75 and p66(mot) in human and murine fibroblast lines, polyclonal antibodies were raised to conserved portions of each sequence. HT1080 and C3H10T1/2 cells were treated with various Grp-inducing agents. A single 75 kDa band was detected by Western blot of cytoplasmic proteins which was not greatly altered after thermal stress or treatment with L-azetidine-2-carboxylic acid or nonactin. However, glucose deprivation by 2-deoxyglucose treatment induced five novel isoforms at 74-75 kDa mass. Mortalin at 66 kDa could not be detected under these treatment conditions. (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码：185 / 190
页数：6
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