Cocoon-Like Self-Degradable DNA Nanoclew for Anticancer Drug Delivery

被引：288

作者：

Sun, Wujin ^{[1
,2
,3
,4
]}

Jiang, Tianyue ^{[1
,2
,3
,4
,5
,6
]}

Lu, Yue ^{[1
,2
,3
,4
]}

Reiff, Margaret ^{[1
,2
]}

Mo, Ran ^{[1
,2
,3
,4
,5
,6
]}

Gu, Zhen ^{[1
,2
,3
,4
]}

机构：

[1] Univ North Carolina Chapel Hill, Joint Dept Biomed Engn, Raleigh, NC 27695 USA

[2] N Carolina State Univ, Raleigh, NC 27695 USA

[3] Univ N Carolina, UNC Eshelman Sch Pharm, Div Mol Pharmaceut, Chapel Hill, NC 27599 USA

[4] Univ N Carolina, UNC Eshelman Sch Pharm, Ctr Nanotechnol Drug Delivery, Chapel Hill, NC 27599 USA

[5] China Pharmaceut Univ, Ctr Drug Discovery, State Key Lab Nat Med, Nanjing 210009, Jiangsu, Peoples R China

[6] China Pharmaceut Univ, Jiangsu Key Lab Drug Discovery Metab Dis, Nanjing 210009, Jiangsu, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2014年 / 136卷 / 42期

关键词：

RELEASE; NANOPARTICLES; NANOFLOWERS; NANOTUBES; SYSTEM;

D O I：

10.1021/ja5088024

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A bioinspired cocoon-like anticancer drug delivery system consisting of a deoxyribonuclease (DNase)-degradable DNA nanoclew (NCl) embedded with an acid-responsive DNase I nanocapsule (NCa) was developed for targeted cancer treatment. The NCl was assembled from a long-chain single-stranded DNA synthesized by rolling-circle amplification (RCA). Multiple GC-pair sequences were integrated into the NCl for enhanced loading capacity of the anticancer drug doxorubicin (DOX). Meanwhile, negatively charged DNase I was encapsulated in a positively charged acid-degradable polymeric nanogel to facilitate decoration of DNase I into the NCl by electrostatic interactions. In an acidic environment, the activity of DNase I was activated through the acid-triggered shedding of the polymeric shell of the NCa, resulting in the cocoon-like self-degradation of the NCl and promoting the release of DOX for enhanced therapeutic efficacy.

引用

页码：14722 / 14725

页数：4

共 30 条

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