Studies on interactions between plant secondary metabolites and glutathione transferase using fluorescence quenching method

被引：0

作者：

Zhang, Xian ^{[1
]}

Cheng, Xinsheng ^{[1
]}

Wang, Chuanqin ^{[1
]}

Xue, Zechun ^{[1
]}

Yang, Liwen ^{[1
]}

Xi, Zheng ^{[1
]}

机构：

[1] Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China

来源：

INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS | 2007年 / 44卷 / 02期

关键词：

plant secondary metabolites; glutathione transferase; fluorescence quenching; UV-Vis absorption spectroscopy;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interactions between plant secondary metabolites (tannic acid, rutin, cinnamic acid and catechin) and gluthathione transferase (GST) were investigated by fluorescence and UV-Vis absorption spectroscopy. Intrinsic fluorescence of GST was measured by selectively exciting their tryptophan (Trp) residues and quenching constants were determined using the Stern-Volmer equation. The binding affinity was found to be strongest for tannic acid and ranked in the order tannic acid > rutin > cinnamic acid > catechin. The pH values in the range of 6.7-7.9, except for tannic acid, did not affect significantly the affinity of rutin, cinnamic acid and catechin with GST. Results showed that the fluorescence quenching of GST was a static_quenching. Fluorescence quenching and UV-Vis absorption spectroscopy suggested that only the tannic acid changed the microenvironrnent of the Trp residues. Furthermore, the number of binding sites and binding constants at different pH values showed that tannic acid had strongest affinity towards GST and hydrogen bonding played an important role in the affinity between GST and the metabolites.

引用

页码：101 / 105

页数：5

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