Exploring the interactions between engineered nanomaterials and immune cells at 3D nano-bio interfaces to discover potent nano-adjuvants

被引：13

作者：

Ma, Ronglin ^{[1
]}

Zheng, Huizhen ^{[1
]}

Liu, Qi ^{[2
]}

Wu, Di ^{[1
]}

Li, Wei ^{[1
]}

Xu, Shujuan ^{[1
]}

Cai, Xiaoming ^{[3
]}

Li, Ruibin ^{[1
]}

机构：

[1] Soochow Univ, State Key Lab Radiat Med & Protect, Sch Radiol & Interdisciplinary Sci RAD X, Collaborat Innovat Ctr Radiol Med,Jiangsu Higher, Suzhou, Jiangsu, Peoples R China

[2] Chinese Acad Agr Sci, Natl Engn Lab Crop Efficient Water Use & Disaster, Key Lab Prevent & Control Residual Pollut Agr Fil, Minist Agr & Rural Affairs,Inst Environm & Sustai, Beijing, Peoples R China

[3] Soochow Univ, Jiangsu Key Lab Prevent & Translat Med Geriatr Di, Ctr Genet Epidemiol & Genom, Sch Publ Hlth, Suzhou, Jiangsu, Peoples R China

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2019年 / 21卷

基金：

中国国家自然科学基金;

关键词：

Adjuvant; 3D culture; Inflammation; Structure-activity relationship; Transformation; GOLD NANOPARTICLES; CARBON NANOTUBES; IN-VITRO; SIZE; INFLAMMASOME; FABRICATION; VACCINES; CHARGE; SHAPE;

D O I：

10.1016/j.nano.2019.102037

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Engineered nanomaterials (ENMs) as adjuvants can potentiate the adaptive immune responses to antigens by activating immune cells in three dimensional (3D) matrixes of tissues. However, few reports explored the interactions of nano-adjuvants and immune cells at 3D nano-bio interfaces. Herein we designed an alginate-calcium microsphere of macrophage cells to explore the interactions. By an extensive comparison of ENM-induced cytokines in 2D and 3D cultured cells, IL-1 beta released in 3D microspheres was found to be a predictive biomarker to assess ENM-induced immune responses in vivo. Among nine representative ENMs, La2O3 boosts the highest adaptive humoral immune response, even stronger than clinically used Alum adjuvant. It could be attributed to the biotransformation of La2O3 from spherical particles into urchin-like LaPO4, resulting in strong biopersistence and NLRP3 inflammasome activation. These findings could be potentially used for the high throughput screening of nano-adjuvants from increasingly invented ENMs to speed up their clinical uses. (C) 2019 Elsevier Inc. All rights reserved.

引用

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