A highly selective ESIPT-based fluorescent probe with a large Stokes shift for the turn-on detection of cysteine and its application in living cells

被引：82

作者：

Sheng, Huace ^{[1
]}

Hu, Yonghong ^{[1
,2
]}

Zhou, Yi ^{[3
]}

Fan, Shimin ^{[2
]}

Cao, Yang ^{[4
]}

Zhao, Xinxin ^{[1
]}

Yang, Wenge ^{[1
]}

机构：

[1] Nanjing Tech Univ, Sch Pharmaceut Sci, 30 South Puzhu Rd, Nanjing 211816, Jiangsu, Peoples R China

[2] Nanjing Tech Univ, Synerget Innovat Ctr Adv Mat, 30 South Puzhu Rd, Nanjing 211816, Jiangsu, Peoples R China

[3] Nanjing Tech Univ, Coll Chem & Mol Engn, Nanjing 211816, Jiangsu, Peoples R China

[4] Jiangsu Prov Hosp, Nanjing 210000, Jiangsu, Peoples R China

来源：

DYES AND PIGMENTS | 2019年 / 160卷

基金：

中国国家自然科学基金;

关键词：

Cysteine; Fluorescent probe; Michael addition; Imidazo[1,5-alpha]pyridine; Cell imaging; AGGREGATION-INDUCED EMISSION; POLYMERIC NANOPARTICLES; ORGANIC NANOPARTICLES; LIQUID-CHROMATOGRAPHY; HYDROGEN-SULFIDE; CLICK REACTION; HUMAN PLASMA; AIE; HOMOCYSTEINE; THIOLS;

D O I：

10.1016/j.dyepig.2018.07.036

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A new imidazo [1,5-alpha]pyridine-based fluorescent probe, named MZC-AC, is developed for the detection of cysteine (Cys) based on an excited state intramolecular proton transfer (ESIPT) mechanism. The probe MZC-AC uses an acrylate moiety as the ESIPT blocking group as well as the recognition site. Upon the treatment with Cys, MZC-AC exhibits a dramatic fluorescence enhancement (85-fold) and a large Stokes shift (166 nm). The limit of detection obtained from fluorescent titration was as low as 0.07 mu M. MZC-AC displays highly sensitive and selective response to Cys over homocysteine (Hcy) and glutathione (GSH). Significantly, MZC-AC could be used to detect Cys in living cells.

引用

页码：48 / 57

页数：10

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