Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle

被引:132
作者
Agrawal, RK
Spahn, CMT
Penczek, P
Grassucci, RA
Nierhaus, KH
Frank, J
机构
[1] Howard Hughes Med Inst Hlth Res Inc, Wadsworth Ctr, Albany, NY 12201 USA
[2] SUNY Albany, Dept Biomed Sci, Wadsworth Ctr, Albany, NY 12201 USA
[3] Max Planck Inst Mol Genet, D-14195 Berlin, Germany
关键词
ribosomes; protein synthesis; tRNA-binding sites; cryoelectron microscopy; elongation cycle;
D O I
10.1083/jcb.150.3.447
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-Angstrom resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible.
引用
收藏
页码:447 / 459
页数:13
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