Efficient and Inducible Use of Artificial MicroRNAs in Marchantia polymorpha

被引：69

作者：

Flores-Sandoval, Eduardo ^{[1
]}

Dierschke, Tom ^{[1
]}

Fisher, Tom J. ^{[1
]}

Bowman, John L. ^{[1
,2
]}

机构：

[1] Monash Univ, Sch Biol Sci, Melbourne, Vic 3800, Australia

[2] Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA

来源：

PLANT AND CELL PHYSIOLOGY | 2016年 / 57卷 / 02期

基金：

澳大利亚研究理事会;

关键词：

Artificial microRNA; AUXIN RESPONSE FACTOR; ENHANCER OF ZESTE; Marchantia; MicroRNA; RESPONSE REGULATOR; Reverse genetics; B RESPONSE REGULATORS; CYTOKININ SIGNAL-TRANSDUCTION; GENE-EXPRESSION; PHYSCOMITRELLA-PATENS; SMALL RNAS; ARABIDOPSIS; SYSTEM; ARGONAUTE; ROLES; TOOL;

D O I：

10.1093/pcp/pcv068

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We describe the efficient use of artificial microRNAs (amiRs) in Marchantia polymorpha using both endogenous and heterologous primary microRNA (pri-miR) hairpin backbones. Targeting of two transcription factor genes, MpARF1 and MpRR-B, mediating different hormonal responses, demonstrated that amiRs can create specific and reproducible physiological and morphological defects, facilitating interpretation of gene function. A third amiR was designed to target a gene encoding a component of the Polycomb recessive complex 2, MpE(z), and constitutive expression of this amiR results in sporeling lethality. Adaptation of an estrogen-inducible system allowed analysis of the phenotypic effects of induction of this amiR during other stages of the life cycle. We discuss the advantages and challenges of the use of amiRs as a tool for reverse genetic analysis in M. polymorpha.

引用

页码：281 / 290

页数：10

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