Immunohistochemical analysis of BRCA2 expression in normal human buccal cells

被引：0
|
作者：
Byrne, TJ
Cohn, GM [1 ]
Lane, MA
Adams, LA
Hoffman, DE
Reece, MT
机构：
[1] Tufts Univ, Sch Med, Baystate Med Ctr, Div Clin & Reprod Genet,Dept Obstet & Gynecol, Springfield, MA 01199 USA
[2] Tufts Univ, Sch Med, Baystate Med Ctr, Womens Hlth Res Lab,Dept Obstet & Gynecol, Springfield, MA 01199 USA
[3] Univ Massachusetts, Dept Plant & Soil Sci, Amherst, MA 01003 USA
来源：
ONCOLOGY REPORTS | 2000年 / 7卷 / 05期
关键词：
BRCA2; immunohistochemistry; buccal cells;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Previously we demonstrated that protein coded by the BRCA1 gene was expressed in normal human buccal cells. The present study confirmed that BRCA2 protein was similarly expressed in these cells. Messenger RNA for BRCA2 was detected with sequential use of two primer sets. Pooled cell samples from healthy donors reacted strongly with two commercially available antibodies, I17 and C15. Immunoreactivity was present in both cytoplasmic and nuclear compartments. We conclude buccal cells will provide a suitable model for exploration of normal BRCA function.
引用
收藏
页码：955 / 958
页数：4
相关论文
共 50 条
  • [41] Immunohistochemical Detection of BRCA-1 and BRCA-2 Expression in Human Breast and Ovarian Tumors
    Vorrius, Thomas R.
    Snyder, Karen
    Pica-Mendez, Arnaldo
    Tan, Charles
    Laterza, Omar
    Toniatti, Carlo
    Carpenter, Chris
    Lee, Hyerim
    Tanaka, Wesley
    Zhang, Zhi-Qiang
    JOURNAL OF HISTOTECHNOLOGY, 2009, 32 (04) : 202 - 203
  • [42] Immunohistochemical analysis of Fas ligand expression in normal human tissues
    Lee, SH
    Shin, MS
    Park, WS
    Kim, SY
    Dong, SM
    Lee, HK
    Park, JY
    Oh, RR
    Jang, JJ
    Lee, JY
    Yoo, NJ
    APMIS, 1999, 107 (11) : 1013 - 1019
  • [43] Expression of human BRCA1 and BRCA2 proteins in lung from a fetus at 19 weeks' gestation
    Bernard-Gallon, DJ
    Dechelotte, P
    Rio, PG
    Bignon, YJ
    INTERNATIONAL JOURNAL OF CANCER, 1999, 82 (05) : 771 - 773
  • [44] iPSCs generation from genetically-corrected Brca2 deficient cells (Brca2Δ27/Δ27)
    Moleiro, V.
    Molina-Estevez, F. J.
    Lozano, M. L.
    Chinchon, R.
    Almarza, E.
    Quintana-Bustamante, O.
    Rio, P.
    Segovia, J. C.
    Gueenechea, G.
    Bueren, J. A.
    Navarro, S.
    HUMAN GENE THERAPY, 2013, 24 (12) : A108 - A108
  • [45] Expression pattern of DNA double strand break repair proteins BRCA1, BRCA2, ATM and RAD51 in normal human tissues
    Barker, L
    Dattani, M
    Duncan, D
    Gray, S
    Quirke, P
    Grabsch, H
    JOURNAL OF PATHOLOGY, 2004, 204 : 48A - 48A
  • [46] Genomic organization and expression of the mouse Brca2 gene
    Nathalie Callens
    Martine Dumont
    Agnès Begue
    Carine Lint
    Jean-Luc Baert
    Jacques Simard
    Yvan Launoit
    Mammalian Genome, 2002, 13 (7) : 352 - 358
  • [47] Quantitative analysis of BRCA1, BRCA2 and hmsh2 mRNA expression in colorectal Lieberkuhnien adenocarcinomas and matched normal mucosa:: Relationship with cellular proliferation
    Le Corre, L
    Vissac-Sabatier, C
    Chalabi, N
    Bignon, YJ
    Daver, A
    Chasseven, A
    Bernard-Gallon, DJ
    ANTICANCER RESEARCH, 2005, 25 (3B) : 2009 - 2016
  • [48] Thymic model for examining BRCA2 expression and function
    Flores, KG
    McAllister, KA
    Greer, PK
    Wiseman, RW
    Hale, LP
    MOLECULAR CARCINOGENESIS, 2002, 35 (03) : 103 - 109
  • [49] Genomic organization and expression of the mouse Brca2 gene
    Callens, N
    Dumont, M
    Begue, A
    Van Lint, C
    Baert, JL
    Simard, J
    de Launoit, Y
    MAMMALIAN GENOME, 2002, 13 (07) : 352 - 358
  • [50] Altered expression of BRCA1, BRCA2, and a newly identified BRCA2 exon 12 deletion variant in malignant human ovarian, prostate, and breast cancer cell lines
    Rauh-Adelmann, C
    Lau, KM
    Sabeti, N
    Long, JP
    Mok, SC
    Ho, SM
    MOLECULAR CARCINOGENESIS, 2000, 28 (04) : 236 - 246
12345