Immunohistochemical analysis of BRCA2 expression in normal human buccal cells

被引：0
|
作者：
Byrne, TJ
Cohn, GM [1 ]
Lane, MA
Adams, LA
Hoffman, DE
Reece, MT
机构：
[1] Tufts Univ, Sch Med, Baystate Med Ctr, Div Clin & Reprod Genet,Dept Obstet & Gynecol, Springfield, MA 01199 USA
[2] Tufts Univ, Sch Med, Baystate Med Ctr, Womens Hlth Res Lab,Dept Obstet & Gynecol, Springfield, MA 01199 USA
[3] Univ Massachusetts, Dept Plant & Soil Sci, Amherst, MA 01003 USA
来源：
ONCOLOGY REPORTS | 2000年 / 7卷 / 05期
关键词：
BRCA2; immunohistochemistry; buccal cells;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Previously we demonstrated that protein coded by the BRCA1 gene was expressed in normal human buccal cells. The present study confirmed that BRCA2 protein was similarly expressed in these cells. Messenger RNA for BRCA2 was detected with sequential use of two primer sets. Pooled cell samples from healthy donors reacted strongly with two commercially available antibodies, I17 and C15. Immunoreactivity was present in both cytoplasmic and nuclear compartments. We conclude buccal cells will provide a suitable model for exploration of normal BRCA function.
引用
收藏
页码：955 / 958
页数：4
相关论文
共 50 条
  • [21] Incorporating tumor immunohistochemical markers in BRCA1 and BRCA2 carrier prediction
    Tai, Yu Chuan
    Chen, Sining
    Parmigiani, Giovanni
    Klein, Alison P.
    BREAST CANCER RESEARCH, 2008, 10 (02):
  • [22] CONDITIONAL BRCA2 SWITCH IN HUMAN CELLS TO STUDY TUMOR PROGRESSION
    Jimenez-Sainz, J.
    Jensen, R. B.
    CLINICAL CANCER RESEARCH, 2019, 25 (22) : 96 - 96
  • [23] Analysis of an estrogen-mediated BRCA2 gene expression mechanism
    Sato, Ami
    Takaoka, Miho
    Saito, Hiroko
    Nakanishi, Akira
    Miki, Yoshio
    CANCER SCIENCE, 2018, 109 : 72 - 72
  • [24] Expression profile of BRCA1 and BRCA2 genes in premenopausal Mexican women with breast cancer: clinical and immunohistochemical correlates
    Gloria Loredo-Pozos
    Erwin Chiquete
    Antonio Oceguera-Villanueva
    Arturo Panduro
    Fernando Siller-López
    Martha E. Ramos-Márquez
    Medical Oncology, 2009, 26 : 269 - 275
  • [25] Expression profile of BRCA1 and BRCA2 genes in premenopausal Mexican women with breast cancer: clinical and immunohistochemical correlates
    Loredo-Pozos, Gloria
    Chiquete, Erwin
    Oceguera-Villanueva, Antonio
    Panduro, Arturo
    Siller-Lopez, Fernando
    Ramos-Marquez, Martha E.
    MEDICAL ONCOLOGY, 2009, 26 (03) : 269 - 275
  • [26] Degradation of BRCA2 expression by hyperthermia sensitizes HRD-negative (BRCA2 wild-type) ovarian cancer cells to niraparib
    Li, J.
    Mei, B.
    Mei, H. J.
    He, S.
    Zhu, Y.
    Huang, J.
    Wang, D.
    Zhang, G.
    ANNALS OF ONCOLOGY, 2022, 33 : S1508 - S1508
  • [27] IMMUNOHISTOCHEMICAL STUDIES OF NEUROCHEMICAL MARKERS IN NORMAL HUMAN BUCCAL MUCOSA
    HILLIGES, M
    HELLMAN, M
    AHLSTROM, U
    JOHANSSON, O
    HISTOCHEMISTRY, 1994, 101 (04) : 235 - 244
  • [28] Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
    Moro, Loredana
    Guaragnella, Nicoletta
    Giannattasio, Sergio
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2015, (102): : 1 - 7
  • [29] Comprehensive Genomic Analysis of a BRCA2 Deficient Human Pancreatic Cancer
    Barber, Louise J.
    Rosa, Juan M. Rosa
    Kozarewa, Iwanka
    Fenwick, Kerry
    Assiotis, Ioannis
    Mitsopoulos, Costas
    Sims, David
    Hakas, Jarle
    Zvelebil, Marketa
    Lord, Christopher J.
    Ashworth, Alan
    PLOS ONE, 2011, 6 (07):
  • [30] Wild type BRCA2 mRNA splice variants in normal and breast tumor cells
    Yoshimatsu, Toshio F.
    Fackenthal, James D.
    Olopade, Olufunmilayo I.
    CANCER RESEARCH, 2011, 71
12345