Immunohistochemical analysis of BRCA2 expression in normal human buccal cells

被引：0
|
作者：
Byrne, TJ
Cohn, GM [1 ]
Lane, MA
Adams, LA
Hoffman, DE
Reece, MT
机构：
[1] Tufts Univ, Sch Med, Baystate Med Ctr, Div Clin & Reprod Genet,Dept Obstet & Gynecol, Springfield, MA 01199 USA
[2] Tufts Univ, Sch Med, Baystate Med Ctr, Womens Hlth Res Lab,Dept Obstet & Gynecol, Springfield, MA 01199 USA
[3] Univ Massachusetts, Dept Plant & Soil Sci, Amherst, MA 01003 USA
来源：
ONCOLOGY REPORTS | 2000年 / 7卷 / 05期
关键词：
BRCA2; immunohistochemistry; buccal cells;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Previously we demonstrated that protein coded by the BRCA1 gene was expressed in normal human buccal cells. The present study confirmed that BRCA2 protein was similarly expressed in these cells. Messenger RNA for BRCA2 was detected with sequential use of two primer sets. Pooled cell samples from healthy donors reacted strongly with two commercially available antibodies, I17 and C15. Immunoreactivity was present in both cytoplasmic and nuclear compartments. We conclude buccal cells will provide a suitable model for exploration of normal BRCA function.
引用
收藏
页码：955 / 958
页数：4
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