Suspension array with shape-coded silica nanotubes for multiplexed immunoassays

被引：66

作者：

He, Bo ^{[1
]}

Son, Sang Jun ^{[1
]}

Lee, Sang Bok ^{[1
]}

机构：

[1] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA

来源：

ANALYTICAL CHEMISTRY | 2007年 / 79卷 / 14期

关键词：

D O I：

10.1021/ac0704964

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A suspension array for multiplexed immunoassays has been developed using shape-coded silica nanotubes (SNTs) as coding materials. Fabricated by multistep anodization template synthesis, each shape-coded SNT has several segments with different reflectance values depending on their diameters and wall thicknesses. Therefore, the code of each SNT can be "read-out" under a conventional optical microscope. The suspension array with shape-coded SNTs has shown high stability and dispersibility in aqueous buffer media and high detection sensitivity. The SNTs have not shown any visible degradation while submerged in aqueous solution for 7 months, the tubular structure and silanol groups on the inner and outer surfaces allow SNTs to disperse evenly in buffer solution, and the detection limit of an IgG protein is about 6 pM with 1.5 x 10(6) SNTs per mL. We have demonstrated the high selectivity of the SNTs suspension array for the detection of multianalytes in the multiplexed immunoassay experiments.

引用

页码：5257 / 5263

页数：7

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