Structural characterization and conformational properties of a polysaccharide isolated from Dendrobium nobile Lindl.

被引：35

作者：

Zhang, Yi ^{[1
,2
,3
,4
]}

Wang, Hongxin ^{[1
,3
]}

Guo, Qingbin ^{[5
]}

Wang, Junqiao ^{[6
]}

Cui, Steve W. ^{[4
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[2] Shanghai Acad Agr Sci, Res Ctr Agr Prod Preservat & Proc, Shanghai 201403, Peoples R China

[3] Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi 214122, Jiangsu, Peoples R China

[4] Agr & Agri Food Canada, Guelph Res & Dev Ctr, 93 Stone Rd West, Guelph, ON N1G 5C9, Canada

[5] Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Tianjin 300222, Peoples R China

[6] Nanchang Univ, State Key Lab Food Sci & Technol, 235 Nanjing East Rd, Nanchang 330047, Jiangxi, Peoples R China

来源：

FOOD HYDROCOLLOIDS | 2020年 / 98卷

关键词：

Dendrobium nobile Lindl; Polysaccharide; Structure; Methylation analysis; NMR; Modelling; O-ACETYL-GLUCOMANNAN; STEMS; NMR; GALACTOGLUCOMANNAN;

D O I：

10.1016/j.foodhyd.2019.01.044

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A water-soluble polysaccharide (JCP-40) from Dendrobium nobile Lindl. was extracted and purified. It was demonstrated as a single symmetrical peak based on HPSEC analysis and had an average molecular weight of 67.5 kDa. Combined with monosaccharide composition analysis, methylation analysis, 1D/2D NMR spectroscopy, the structural features of JCP-40 was proposed as follows: the major sugar residues of JCP-40 consisted of -> 4)-beta-D-Manp (1 -> and -> 4)-beta-D-Glcp (1 ->. Acetyl groups were found attached to the O-2 or O-3 positions on mannosyl residues based on NMR spectrum results. Rotational isomeric state (RIS)-Metropolis Monte Carlo method indicated that repeating unit increased from 30 to 120, the influence of Mw in this range affected chain flexibility. The conformational parameters (derived from R-g vs Mw and [eta] vs Mw) from HPSEC suggested a random coil conformation for JCP-40.

引用

页数：10

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