The exosome: A conserved eukaryotic RNA processing complex containing multiple 3'->5' exoribonucleases

被引：775

作者：

Mitchell, P ^{[1
]}

Petfalski, E ^{[1
]}

Shevchenko, A ^{[1
]}

Mann, M ^{[1
]}

Tollervey, D ^{[1
]}

机构：

[1] EUROPEAN MOL BIOL LAB,EMBL,D-69012 HEIDELBERG,GERMANY

来源：

CELL | 1997年 / 91卷 / 04期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/S0092-8674(00)80432-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We identified a complex in S. cerevisiae, the ''exosome,'' consisting of the five essential proteins Rrp4p, Rrp41p, Rrp42p, Rrp43p, and Rrp44p (Dis3p). Remarkably, four of these proteins are homologous to characterized bacterial 3'-->5' exoribonucleases; Rrp44p is homologous to RNase II, while Rrp41p, Rrp42p, and Rrp43p are related to RNase PH. Recombinant Rrp4p, Rrp44p, and Rrp41p are 3'-->5' exoribonucleases in vitro that have distributive, processive, and phosphorolytic activities, respectively. All components of the exosome are required for 3' processing of the 5.8S rRNA. Human Rrp4p is found in a comparably sized complex, and expression of the hRRP4 gene in yeast complements the rrp4-1 mutation. We conclude that the exosome constitutes a highly conserved eukaryotic RNA processing complex.

引用

页码：457 / 466

页数：10

共 48 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] IDENTIFICATION AND FUNCTIONAL-ANALYSIS OF 2 U3 BINDING-SITES ON YEAST PRERIBOSOMAL RNA [J].

BELTRAME, M ;

TOLLERVEY, D .

EMBO JOURNAL, 1992, 11 (04) :1531-1542

[3] The yeast Pan2 protein is required for poly(A)-binding protein-stimulated poly(A)-nuclease activity [J].

Boeck, R ;

Tarun, S ;

Rieger, M ;

Deardorff, JA ;

MullerAuer, S ;

Sachs, AB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (01) :432-438

[4]

Brown CE, 1996, MOL CELL BIOL, V16, P5744

[5] COPURIFICATION OF ESCHERICHIA-COLI RNASE-E AND PNPASE - EVIDENCE FOR A SPECIFIC ASSOCIATION BETWEEN 2 ENZYMES IMPORTANT IN RNA PROCESSING AND DEGRADATION [J].

CARPOUSIS, AJ ;

VANHOUWE, G ;

EHRETSMANN, C ;

KRISCH, HM .

CELL, 1994, 76 (05) :889-900

[6]

Deutscher M P, 1990, Prog Nucleic Acid Res Mol Biol, V39, P209, DOI 10.1016/S0079-6603(08)60628-5

[7] PROMISCUOUS EXORIBONUCLEASES OF ESCHERICHIA-COLI [J].

DEUTSCHER, MP .

JOURNAL OF BACTERIOLOGY, 1993, 175 (15) :4577-4583

[8] ENZYMATIC BASIS FOR HYDROLYTIC VERSUS PHOSPHOROLYTIC MESSENGER-RNA DEGRADATION IN ESCHERICHIA-COLI AND BACILLUS-SUBTILIS [J].

DEUTSCHER, MP ;

REUVEN, NB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (08) :3277-3280

[9]

DIGNAM JD, 1983, METHOD ENZYMOL, V101, P582

[10] THE NOVEL NUCLEAR GENE DSS-1 OF SACCHAROMYCES-CEREVISIAE IS NECESSARY FOR MITOCHONDRIAL BIOGENESIS [J].

DMOCHOWSKA, A ;

GOLIK, P ;

STEPIEN, PP .

CURRENT GENETICS, 1995, 28 (02) :108-112

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