Longitudinal analysis of humoral immunity against SARS-CoV-2 Spike in convalescent individuals up to 8 months post-symptom onset

被引：95

作者：

Anand, Sai Priya ^{[1
,2
]}

Prevost, Jeremie ^{[2
,3
]}

Nayrac, Manon ^{[2
,3
]}

Beaudoin-Bussieres, Guillaume ^{[2
,3
]}

Benlarbi, Mehdi ^{[2
]}

Gasser, Romain ^{[2
,3
]}

Brassard, Nathalie ^{[2
]}

Laumaea, Annemarie ^{[2
,3
]}

Gong, Shang Yu ^{[1
,2
]}

Bourassa, Catherine ^{[2
]}

Brunet-Ratnasingham, Elsa ^{[2
,3
]}

Medjahed, Halima ^{[2
]}

Gendron-Lepage, Gabrielle ^{[2
]}

Goyette, Guillaume ^{[2
]}

Gokool, Laurie ^{[2
]}

Morrisseau, Chantal ^{[2
]}

Begin, Philippe ^{[2
,5
]}

Martel-Laferriere, Valerie ^{[2
,3
]}

Tremblay, Cecile ^{[2
,3
]}

Richard, Jonathan ^{[2
,3
]}

Bazin, Renee ^{[4
]}

Duerr, Ralf ^{[6
,7
]}

Kaufmann, Daniel E. ^{[2
,8
]}

Finzi, Andres ^{[1
,2
,3
]}

机构：

[1] McGill Univ, Dept Microbiol & Immunol, Montreal, PQ H3A 2B4, Canada

[2] CHUM, Ctr Rech, Montreal, PQ H2X 0A9, Canada

[3] Univ Montreal, Dept Microbiol Infectiol & Immunol, Montreal, PQ H2X 0A9, Canada

[4] Hema Quebec, Affaires Med & Innovat, Quebec City, PQ G1V 5C3, Canada

[5] CHU Ste Justine, Montreal, PQ H3T 1C5, Canada

[6] NYU, Dept Pathol, Sch Med, New York, NY 10016 USA

[7] NYU, Dept Microbiol, Sch Med, New York, NY 10016 USA

[8] Univ Montreal, Dept Med, Montreal, PQ H3T 1J4, Canada

来源：

CELL REPORTS MEDICINE | 2021年 / 2卷 / 06期

基金：

加拿大健康研究院; 加拿大创新基金会;

关键词：

EVOLUTION; MILD;

D O I：

10.1016/j.xcrm.2021.100290

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

With the recent approval of highly effective coronavirus disease 2019 (COVID-19) vaccines, functional and lasting immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently under investigation as antibody levels in plasma were shown to decline during convalescence. Since the absence of antibodies does not equate to absence of immune memory, we evaluate the presence of SARS-CoV-2-specific memory B cells in convalescent individuals. Here, we report a longitudinal assessment of humoral immune responses on 32 donors up to 8 months post-symptom onset. Our observations indicate that anti-Spike and anti-receptor binding domain (RBD) immunoglobulin M (IgM) in plasma decay rapidly, whereas the reduction of IgG is less prominent. Neutralizing activity also declines rapidly when compared to Fc-effector functions. Concomitantly, the frequencies of RBD-specific IgM+ B cells wane significantly when compared to RBD-specific IgG+ B cells, which remain stable. Our results add to the current understanding of immune memory following SARS-CoV-2 infection, which is critical for secondary infection prevention and vaccine efficacy.

引用

页数：15

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