MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus

被引:38
作者
Hezova, Renata [1 ,3 ]
Kovarikova, Alena [1 ]
Srovnal, Josef [2 ]
Zemanova, Milada [4 ]
Harustiak, Tomas [5 ]
Ehrmann, Jiri [2 ]
Hajduch, Marian [2 ]
Sachlova, Milana [3 ]
Svoboda, Marek [3 ]
Slaby, Ondrej [1 ,3 ]
机构
[1] Masaryk Univ, Cent European Inst Technol CEITEC, Kamenice 5, Brno 62500, Czech Republic
[2] Palacky Univ, Fac Med, CR-77147 Olomouc, Czech Republic
[3] Masaryk Mem Canc Inst, Dept Comprehens Canc Care, Brno, Czech Republic
[4] Gen Univ Hosp Prague, Dept Oncol, Prague, Czech Republic
[5] Univ Hosp Motol, Dept Surg, Prague, Czech Republic
关键词
Esophageal adenocarcinoma; Esophageal squamous cell carcinoma; miR-205; Tumor suppressor; Oncogene; MICRORNA EXPRESSION; BREAST-CANCER; NODE METASTASIS; PROLIFERATION; PROMOTES; PROGRESSION; PROFILES; MELANOMA; PROSTATE; INVASION;
D O I
10.1007/s13277-015-4656-8
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Esophageal cancer is a malignant disease with poor prognosis, increasing incidence, and ineffective treatment options. MicroRNAs are post-transcriptional regulators of gene expression involved in many biological processes including carcinogenesis. We determined miR-205 expression levels in tumor/non-tumor tissues of 45 esophageal cancer patients using qPCR and found that decreased level of miR-205 in tumor tissue correlates with poor overall survival in esophageal adenocarcinoma patients. Further, we observed significantly higher levels of miR-205 in tumor tissue of esophageal squamous cell carcinoma. Ectopic overexpression of miR-205 in adenocarcinoma cell line SK-GT-4 led to decreased cell proliferation, cell cycle arrest in G1, and decreased migration ability. Conversely, in squamous cell line KYSE-150, same effects like inhibition of proliferation, migration, and colony-forming potential and cell cycle arrest in G2 were observed after silencing of miR-205. We performed global gene expression profiling and revealed that suppressive functioning of miR-205 in adenocarcinoma could be realized through regulation of epithelial-mesenchymal transition (EMT), whereas oncogenic in squamous cell carcinoma by regulation of metalloproteinase 10. Our results suggest that miR-205 could serve as biomarker in esophageal cancer and acts as a tumor suppressor in esophageal adenocarcinoma and oncogene in esophageal squamous cell carcinoma.
引用
收藏
页码:8007 / 8018
页数:12
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