Long-term suspension culture of Triticum timopheevii cells: 2. Kinetics of cell population

The size of the proliferative pool and the parameters of mitotic cycle (MC) were evaluated by the autoradiographic method in a rapidly growing long-term suspension culture of Triticum timopheevii (Zhuk.) Zhuk. cells. The proliferative pool reached 95%, MC was 17.5-h-long, and the mitosis itself lasted for 1 h. These temporal characteristics approached values typical of the apical meristematic cells in T. timopheevii root tips. However, other MC phases changed significantly: the G(1) period was reduced almost completely and the S period was shortened twofold (to 2.5 h), whereas the G(2) period expanded twofold (to 14.03 h). The correlation between the kinetics of cell proliferation and the growth pattern in a long-subcultivated suspension culture of T. timopheevii is discussed.