Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids

被引：137

作者：

Drienovska, Ivana ^{[1
]}

Rioz-Martinez, Ana ^{[1
]}

Draksharapu, Apparao ^{[1
]}

Roelfes, Gerard ^{[1
]}

机构：

[1] Univ Groningen, Stratingh Inst Chem, NL-9747 AG Groningen, Netherlands

来源：

CHEMICAL SCIENCE | 2015年 / 6卷 / 01期

基金：

欧洲研究理事会;

关键词：

DUAL ANCHORING STRATEGY; GENETIC INCORPORATION; SITE; PROTEIN; ION; METALLOPROTEIN; HYDROGENATION; STREPTAVIDIN; PURIFICATION; COMPLEXES;

D O I：

10.1039/c4sc01525h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Artificial metalloenzymes have emerged as an attractive new approach to enantioselective catalysis. Herein, we introduce a novel strategy for preparation of artificial metalloenzymes utilizing amber stop codon suppression methodology for the in vivo incorporation of metal-binding unnatural amino acids. The resulting artificial metalloenzymes were applied in catalytic asymmetric Friedel-Crafts alkylation reactions and up to 83% ee for the product was achieved.

引用

页码：770 / 776

页数：7