Age-related effects of TGF-β on proteoglycan synthesis in equine articular cartilage

被引：40
|
作者：
Iqbal, J [1 ]
Dudhia, J [1 ]
Bird, JLE [1 ]
Bayliss, MT [1 ]
机构：
[1] Univ London Royal Vet Coll, Dept Vet Basic Sci & Farm Anim & Equine Med & Sur, London NW1 0TU, England
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 274卷 / 02期
关键词：
cartilage; TGF-beta; proteoglycan; mRNA;
D O I：
10.1006/bbrc.2000.3167
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The synthesis of proteoglycans was measured in normal equine articular cartilage of ages 9 months to 20 years and the effect of TGF-beta 1 on this activity was investigated. The rate of incorporation of [S-35]Na2SO4 decreased with age as did the responsiveness of the tissue to the growth factor. The enhanced synthesis of proteoglycan induced at all ages by TGF-beta 1 was down-regulated by IL-1 beta and retinoic acid. The expression of mRNA for TGF-beta 1, 2, and 3 was also measured, and although the level of TGF-beta 1 was highest at all ages, the expression of each growth factor decreased with age. (C) 2000 Academic Press.
引用
收藏
页码：467 / 471
页数：5
相关论文
共 50 条
  • [1] The effect of link peptide on proteoglycan synthesis in equine articular cartilage
    Dean, MF
    Lee, YW
    Dastjerdi, AM
    Lees, P
    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2003, 1622 (03): : 161 - 168
  • [2] Quantification and age-related distribution of articular cartilage degeneration in the equine fetlock joint
    Brommer, H
    van Weeren, RR
    Brama, PAJ
    Barneveld, A
    EQUINE VETERINARY JOURNAL, 2003, 35 (07) : 697 - 701
  • [3] Effects of TGF-β1 on alternative splicing of Superficial Zone Protein in articular cartilage cultures
    DuRaine, G. D.
    Chan, S. M. T.
    Reddi, A. H.
    OSTEOARTHRITIS AND CARTILAGE, 2011, 19 (01) : 103 - 110
  • [4] TGF-β1 Improves Articular Cartilage Damage in Rabbit Knee
    Singh, N. K.
    Shiwani, S.
    Singh, G. R.
    Jeong, D. K.
    Kinjavdekar, P.
    Amarpal
    Lohakare, J. D.
    Lee, S. J.
    PAKISTAN VETERINARY JOURNAL, 2012, 32 (03) : 412 - 417
  • [5] Exercise-induced changes in proteoglycan metabolism of equine articular cartilage
    Bird, JLE
    Platt, D
    Wells, T
    May, SA
    Bayliss, MT
    EQUINE VETERINARY JOURNAL, 2000, 32 (02) : 161 - 163
  • [6] Age-related changes in the synthesis and mRNA expression of decorin and aggrecan in human meniscus and articular cartilage
    McAlinden, A
    Dudhia, J
    Bolton, MC
    Lorenzo, P
    Heinegård, D
    Bayliss, MT
    OSTEOARTHRITIS AND CARTILAGE, 2001, 9 (01) : 33 - 41
  • [7] Age-related expression of molecular regulators of hypertrophy and maturation in articular cartilage
    Semevolos, Stacy A.
    Nixon, Alan J.
    Fortier, Lisa A.
    Strassheim, M. Lisa
    Haupt, Jennifer
    JOURNAL OF ORTHOPAEDIC RESEARCH, 2006, 24 (08) : 1773 - 1781
  • [8] Influence of exercise and joint topography on depth-related spatial distribution of proteoglycan and collagen content in immature equine articular cartilage
    Brama, P. A. J.
    Holopainen, J.
    van Weeren, P. R.
    Firth, E. C.
    Helminen, H. J.
    Hyttinen, M. M.
    EQUINE VETERINARY JOURNAL, 2009, 41 (06) : 557 - 563
  • [9] Tobacco smoke extract induces age-related changes due to modulation of TGF-β
    Yin, L
    Morita, A
    Tsuji, T
    EXPERIMENTAL DERMATOLOGY, 2003, 12 : 51 - 56
  • [10] Effects of electromagnetic fields on proteoglycan metabolism of bovine articular cartilage explants
    De Mattei, M
    Pasello, M
    Pellati, A
    Stabellini, G
    Massari, L
    Gemmati, D
    Caruso, A
    CONNECTIVE TISSUE RESEARCH, 2003, 44 (3-4) : 154 - 159
12345