Long-Term Grow-Out Affects Campylobacter jejuni Colonization Fitness in Coincidence With Altered Microbiota and Lipid Composition in the Cecum of Laying Hens

被引:9
作者
Asakura, Hiroshi [1 ]
Nakayama, Tatsuya [1 ]
Yamamoto, Shiori [1 ]
Izawa, Kazuki [2 ]
Kawase, Jun [3 ]
Torii, Yasushi [4 ]
Murakami, Satoshi [1 ]
机构
[1] Natl Inst Hlth Sci, Div Biomed Food Res, Kawasaki, Kanagawa, Japan
[2] Tokyo Inst Technol, Dept Comp Sci, Meguro, Japan
[3] Shimane Prefectural Inst Publ Hlth & Environm Sci, Dept Bacteriol, Matsue, Shimane, Japan
[4] Tokyo Univ Agr, Dept Anim Hyg, Atsugi, Kanagawa, Japan
基金
日本学术振兴会;
关键词
Campylobacter jejuni; laying hen; long-term breeding; gut microbiota; lipidome; BROILER-CHICKENS; IMMUNE-RESPONSE; INFECTIONS; POULTRY; ACIDS; HOST;
D O I
10.3389/fvets.2021.675570
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Campylobacter jejuni is one of the leading causes of gastrointestinal illness worldwide and is mainly transmitted from chicken through the food chain. Previous studies have provided increasing evidence that this pathogen can colonize and replicate in broiler chicken during its breeding; however, its temporal kinetics in laying hen are poorly understood. Considering the possible interaction between C. jejuni and gut microbiota, the current study was conducted to address the temporal dynamics of C. jejuni in the cecum of laying hen over 40 weeks, with possible alteration of the gut microbiota and fatty acid (FA) components. Following oral infection with C. jejuni 81-176, inocula were stably recovered from ceca for up to 8 weeks post-infection (p.i.). From 16 weeks p.i., most birds became negative for C. jejuni and remained negative up to 40 weeks p.i. 16S rRNA gene sequencing analyses revealed that most of the altered relative rRNA gene abundances occurred in the order Clostridiales, in which increased relative rRNA gene abundances were observed at >16 weeks p.i. in the families Clostridiaceae, Ruminococcaceae, Lachnospiraceae, and Peptococcaceae. Lipidome analyses revealed increased levels of sterols associated with bile acid metabolisms in the cecum at 16 and/or 24 weeks p.i. compared with those detected at 8 weeks p.i., suggesting that altered microbiota and bile acid metabolism might underlie the decreased colonization fitness of C. jejuni in the gut of laying hens.
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页数:13
相关论文
共 60 条
[1]   A Systematic Review Characterizing On-Farm Sources of Campylobacter spp. for Broiler Chickens [J].
Agunos, Agnes ;
Waddell, Lisa ;
Leger, David ;
Taboada, Eduardo .
PLOS ONE, 2014, 9 (08)
[2]   Microbiological Quality Assessment of Game Meats at Retail in Japan [J].
Asakura, Hiroshi ;
Kawase, Jun ;
Ikeda, Tetsuya ;
Honda, Mioko ;
Sasaki, Yoshimasa ;
Uema, Masashi ;
Kabeya, Hidenori ;
Sugiyama, Hiromu ;
Igimi, Shizunobu ;
Takai, Shinji .
JOURNAL OF FOOD PROTECTION, 2017, 80 (12) :2119-2126
[3]   Ex vivo proteomics of Campylobacter jejuni 81-176 reveal that FabG affects fatty acid composition to alter bacterial growth fitness in the chicken gut [J].
Asakura, Hiroshi ;
Kawamoto, Keiko ;
Murakami, Satoshi ;
Tachibana, Masato ;
Kurazono, Hisao ;
Makino, Sou-ichi ;
Yamamoto, Shigeki ;
Igimi, Shizunobu .
RESEARCH IN MICROBIOLOGY, 2016, 167 (02) :63-71
[4]   Age-Related Differences in the Luminal and Mucosa-Associated Gut Microbiome of Broiler Chickens and Shifts Associated with Campylobacter jejuni Infection [J].
Awad, Wageha A. ;
Mann, Evelyne ;
Dzieciol, Monika ;
Hess, Claudia ;
Schmitz-Esser, Stephan ;
Wagner, Martin ;
Hess, Michael .
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2016, 6
[5]   Interactions between the microbiota and pathogenic bacteria in the gut [J].
Baumler, Andreas J. ;
Sperandio, Vanessa .
NATURE, 2016, 535 (7610) :85-93
[6]   Inhibition and Interactions of Campylobacter jejuni from Broiler Chicken Houses with Organic Acids [J].
Beier, Ross C. ;
Byrd, J. Allen ;
Caldwell, Denise ;
Andrews, Kathleen ;
Crippen, Tawni L. ;
Anderson, Robin C. ;
Nisbet, David J. .
MICROORGANISMS, 2019, 7 (08)
[7]  
BOHNHOFF M, 1954, P SOC EXP BIOL MED, V86, P132, DOI 10.3181/00379727-86-21030
[8]   Investigation of the Presence and Protective Effects of Maternal Antibodies Against Campylobacter jejuni in Chickens [J].
Cawthraw, S. A. ;
Newell, D. G. .
AVIAN DISEASES, 2010, 54 (01) :86-93
[9]   Bile acids: regulation of synthesis [J].
Chiang, John Y. L. .
JOURNAL OF LIPID RESEARCH, 2009, 50 (10) :1955-1966
[10]   Metabolic and hormonal responses of growing modern meat-type chickens to fasting [J].
Christensen, K. ;
McMurtry, J. P. ;
Thaxton, Y. V. ;
Thaxton, J. P. ;
Corzo, A. ;
McDaniel, C. ;
Scanes, C. G. .
BRITISH POULTRY SCIENCE, 2013, 54 (02) :199-205