Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar

被引：1

作者：

Touloudi, Antonia ^{[1
]}

Valiakos, George ^{[1
]}

Cawthraw, Shaun ^{[2
]}

Kostoulas, Polychronis ^{[1
,3
]}

Gortazar, Christian ^{[4
]}

Boadella, Mariana ^{[5
]}

Giannakopoulos, Alexios ^{[1
]}

Birtsas, Periklis ^{[6
]}

Sofia, Marina ^{[1
]}

Athanasiou, Labrini V. ^{[1
]}

Satra, Maria ^{[3
]}

Athanasakopoulou, Zoi ^{[1
]}

Kantere, Maria ^{[1
]}

Spyrou, Vassiliki ^{[7
]}

Petrovska, Liljana ^{[2
]}

Billinis, Charalambos ^{[1
,3
]}

机构：

[1] Univ Thessaly, Fac Vet Sci, Kardhitsa 43100, Greece

[2] Anim & Plant Hlth Agcy, Addlestone KT15 3NB, Surrey, England

[3] Univ Thessaly, Fac Publ & Integrated Hlth, Kardhitsa 43100, Greece

[4] Inst Invest Recursos Cineget IREC, SaBio, Ciudad Real 13005, Spain

[5] SABIOTEC, Camino Moledores S-N, Ciudad Real 13005, Spain

[6] Fac Forestry Wood Sci & Design, Kardhitsa 43100, Greece

[7] Univ Thessaly, Fac Anim Sci, Larisa 41222, Greece

来源：

MICROORGANISMS | 2021年 / 9卷 / 05期

关键词：

multiplex bead assay; Mycobacterium bovis; Brucella suis; Trichinella spiralis; wild boar; serological assay; LINKED-IMMUNOSORBENT-ASSAY; SUS-SCROFA; TUBERCULOSIS; TRICHINOSIS; DIAGNOSIS; SWINE; EPIDEMIOLOGY; INFECTION; RESPONSES; LIVESTOCK;

D O I：

10.3390/microorganisms9050904

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis. Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronegative), Brucella (30 seropositive, 39 seronegative), and Trichinella (21 seropositive, 97 seronegative) were used for the development and evaluation of the assay. Magnetic beads coated with recombinant MPB83 antigen (TB), a whole-cell B. suis 1330 antigen, and an E/S T. spiralis antigen were used for the detection of specific antibodies using Bio-Rad Bio-Plex technology. The sensitivities (Se) and specificities (Sp) of the multiplex assay were, for M. bovis, 0.98 and 0.86; for B. suis, 1.00 and 0.97; and for T. spiralis, 0.90 and 0.99 (Se and Sp, respectively). The results show the diagnostic potential of this assay for the simultaneous detection of antibodies against M. bovis, B. suis, and T. spiralis in wild boar.

引用

页数：9

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