Dynamics of proteolysis, protease activity and bacterial community of Neolamarckia cadamba leaves silage and the effects of formic acid and Lactobacillus farciminis

被引：49

作者：

He, Liwen ^{[1
]}

Wang, Cheng ^{[1
]}

Xing, Yaqi ^{[1
]}

Zhou, Wei ^{[1
]}

Pian, Ruiqi ^{[1
]}

Yang, Fuyu ^{[1
]}

Chen, Xiaoyang ^{[1
]}

Zhang, Qing ^{[1
]}

机构：

[1] South China Agr Univ, Guangdong Res & Dev Ctr Modern Agr Woody Forage I, Guangdong Key Lab Innovat Dev & Utilizat Forest P, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou, Guangdong, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2019年 / 294卷

关键词：

Neolamarckia cadamba leaf; Protease; Formic acid; Fermentation quality; Bacterial community; ALFALFA; FERMENTATION; NITROGEN; QUALITY; INOCULATION; RYEGRASS; TANNIN;

D O I：

10.1016/j.biortech.2019.122127

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

To investigate the reason for well preservation of protein in Neolamarckia cadamba leaves (NCL) during ensiling, fresh NCL were ensiled with or without addition of 2.0 mL/kg formic acid (FA) or 1.0 x 10(9) CFU/kg Lactobacillus farciminis (LF), and the dynamics of protease activity and microbial community were analyzed. Nonprotein-N, free amino acid, ammonia-N, the activities of carboxypeptidase and aminopeptidase, and bacterial diversity were low during NCL ensiling. Exiguobacterium dominated in NCL silage and its relative abundance increased while Enterobacter abundance decreased during ensiling. FA lowered (P < 0.05) pH and coliform bacteria number, while LF increased (P < 0.05) lactic acid bacteria number, lactic acid content and Lactobacillus abundance at the early stage of fermentation. In summary, protein in NCL can be well preserved during ensiling likely due to its low protease and bacterial activity, and FA and LF improve the quality of NCL silage in different ways.

引用

页数：8

共 42 条

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