Action Potential Initiation in Neocortical Inhibitory Interneurons

被引：81

作者：

Li, Tun ^{[1
,2
,3
]}

Tian, Cuiping ^{[1
,2
,3
]}

Scalmani, Paolo ^{[4
]}

Frassoni, Carolina ^{[5
]}

Mantegazza, Massimo ^{[6
,7
]}

Wang, Yonghong ^{[1
,2
,3
]}

Yang, Mingpo ^{[1
,2
,3
]}

Wu, Si ^{[8
,9
,10
]}

Shu, Yousheng ^{[8
,9
,10
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Neurosci, Shanghai, Peoples R China

[3] Univ Chinese Acad Sci, Shanghai, Peoples R China

[4] Fdn Ist Ric & Cura Carattere Sci IRCCS, UO Neurophysiopathol & Diagnost Epileptol, Neurol Inst Carlo Besta, Milan, Italy

[5] Fdn Ist Ric & Cura Carattere Sci IRCCS, Neurol Inst Carlo Besta, UO Clin Epileptol & Expt Neurophysiol, Milan, Italy

[6] CNRS, UMR7275, Lab Excellence Ion Channel Sci & Therapeut LabEx, IPMC, F-06560 Valbonne, France

[7] Univ Nice Sophia Antipolis, Valbonne, France

[8] Beijing Normal Univ, Sch Brain & Cognit Sci, State Key Lab Cognit Neurosci & Learning, Beijing 100875, Peoples R China

[9] Beijing Normal Univ, Sch Brain & Cognit Sci, IDG McGovern Inst Brain Res, Beijing 100875, Peoples R China

[10] Beijing Normal Univ, Ctr Collaborat & Innovat Brain & Learning Sci, Beijing 100875, Peoples R China

来源：

PLOS BIOLOGY | 2014年 / 12卷 / 09期

基金：

中国国家自然科学基金;

关键词：

CEREBELLAR PURKINJE NEURONS; NEONATAL-INFANTILE SEIZURES; SEVERE MYOCLONIC EPILEPSY; DE-NOVO MUTATIONS; SODIUM-CHANNEL; PYRAMIDAL NEURONS; GABAERGIC INTERNEURONS; DRAVET SYNDROME; ION CHANNELS; MOUSE MODEL;

D O I：

10.1371/journal.pbio.1001944

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Action potential (AP) generation in inhibitory interneurons is critical for cortical excitation-inhibition balance and information processing. However, it remains unclear what determines AP initiation in different interneurons. We focused on two predominant interneuron types in neocortex: parvalbumin (PV)-and somatostatin (SST)-expressing neurons. Patch-clamp recording from mouse prefrontal cortical slices showed that axonal but not somatic Na+ channels exhibit different voltage-dependent properties. The minimal activation voltage of axonal channels in SST was substantially higher (similar to 7mV) than in PV cells, consistent with differences in AP thresholds. A more mixed distribution of high-and low-threshold channel subtypes at the axon initial segment (AIS) of SST cells may lead to these differences. Surprisingly, Na(V)1.2 was found accumulated at AIS of SST but not PV cells; reducing Na(V)1.2-mediated currents in interneurons promoted recurrent network activity. Together, our results reveal the molecular identity of axonal Na+ channels in interneurons and their contribution to AP generation and regulation of network activity.

引用

页数：16

共 60 条

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