Process optimization for the production of high-concentration ethanol with Scenedesmus raciborskii biomass

被引:30
作者
Alam, Md. Asraful [1 ]
Yuan, Tao [2 ]
Xiong, Wenlong [1 ]
Zhang, Beixiao [1 ]
Lv, Yongkun [1 ]
Xu, Jingliang [1 ,2 ]
机构
[1] Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Henan, Peoples R China
[2] Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
关键词
Bioethanol; Enzymatic hydrolysis; Fed-batch; Glucose; Microalgae; BIOETHANOL PRODUCTION; ENZYMATIC-HYDROLYSIS; MICROALGAL BIOMASS; SIMULTANEOUS SACCHARIFICATION; FERMENTATION; PRETREATMENT; BIOFUEL; BIODIESEL; VISCOSITY; STRAW;
D O I
10.1016/j.biortech.2019.122219
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Scenedesmus raciborskii WZKMT was subjected to fed-batch enzymatic hydrolysis and fermentation to facilitate the saccharification of high-solid-loading substrate for high-concentration ethanol. In this work, process factors affecting enzymatic hydrolysis, including enzyme loading, temperature, pH, and solid loading, were optimized. Results showed that 58.03 g L-1 glucose, 12.57 g L-1 xylose, and 1.45 g L-1 cellobiose were obtained after the enzymatic hydrolysis of 330 g L-1 substrates under the optimal conditions of 30 FPU g(-1) enzyme loading, 50 degrees C, and pH 5.5. Meanwhile, 89.60% yield and 30.43 g L-1 content of ethanol were obtained after the fermentation of 330 g L-1 hydrolysate. The maximum ethanol concentration of 79.38 g L-1 could be achieved through repeated fed-batch process, indicating that S. raciborskii WZKMT is a promising feedstock for ethanol production.
引用
收藏
页数:7
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共 38 条
[1]   Microalgae-Novel Highly Efficient Starch Producers [J].
Branyikova, Irena ;
Marsalkova, Barbora ;
Doucha, Jiri ;
Branyik, Tomas ;
Bisova, Katerina ;
Zachleder, Vilem ;
Vitova, Milada .
BIOTECHNOLOGY AND BIOENGINEERING, 2011, 108 (04) :766-776
[2]   Microalgae-based carbohydrates for biofuel production [J].
Chen, Chun-Yen ;
Zhao, Xin-Qing ;
Yen, Hong-Wei ;
Ho, Shih-Hsin ;
Cheng, Chieh-Lun ;
Lee, Duu-Jong ;
Bai, Feng-Wu ;
Chang, Jo-Shu .
BIOCHEMICAL ENGINEERING JOURNAL, 2013, 78 :1-10
[3]   Ternary deep eutectic solvents for effective biomass deconstruction at high solids and low enzyme loadings [J].
Chen, Zhu ;
Jacoby, William A. ;
Wan, Caixia .
BIORESOURCE TECHNOLOGY, 2019, 279 :281-286
[4]   Biodiesel from microalgae beats bioethanol [J].
Chisti, Yusuf .
TRENDS IN BIOTECHNOLOGY, 2008, 26 (03) :126-131
[5]   Hydrothermal acid treatment for sugar extraction from Golenkinia sp. [J].
Choi, Sun-A ;
Choi, Won-Il ;
Lee, Jin-Suk ;
Kim, Seung Wook ;
Lee, Gye-An ;
Yun, Jihyun ;
Park, Ji-Yeon .
BIORESOURCE TECHNOLOGY, 2015, 190 :408-411
[6]   A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].
DUBOIS, M ;
GILLES, K ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
NATURE, 1951, 168 (4265) :167-167
[7]   Long-term production of bioethanol in repeated-batch fermentation of microalgal biomass using immobilized Saccharomyces cerevisiae [J].
El-Dalatony, Marwa M. ;
Kurade, Mayur B. ;
Abou-Shanab, Reda A. I. ;
Kim, Hoo ;
Salama, El-Sayed ;
Jeon, Byong-Hun .
BIORESOURCE TECHNOLOGY, 2016, 219 :98-105
[8]   Biofuel for vehicle use in China: Current status, future potential and policy implications [J].
Hao, Han ;
Liu, Zongwei ;
Zhao, Fuquan ;
Ren, Jingzheng ;
Chang, Shiyan ;
Rong, Ke ;
Du, Jiuyu .
RENEWABLE & SUSTAINABLE ENERGY REVIEWS, 2018, 82 :645-653
[9]   Exploring alkaline pre-treatment of microalgal biomass for bioethanol production [J].
Harun, Razif ;
Jason, W. S. Y. ;
Cherrington, Tamara ;
Danquah, Michael K. .
APPLIED ENERGY, 2011, 88 (10) :3464-3467
[10]   Saccharification of carbohydrates in microalgal biomass by physical, chemical and enzymatic pre-treatments as a previous step for bioethanol production [J].
Hernandez, D. ;
Riano, B. ;
Coca, M. ;
Garcia-Gonzalez, M. C. .
CHEMICAL ENGINEERING JOURNAL, 2015, 262 :939-945