Dynamics of replication-independent histone turnover in budding yeast

被引：436
作者：
Dion, Michael F.
Kaplan, Tommy
Kim, Minkyu
Buratowski, Stephen
Friedman, Nir
Rando, Oliver J.
机构：
[1] Harvard Univ, Fac Arts & Sci, Ctr Syst Biol, Cambridge, MA 02138 USA
[2] Hebrew Univ Jerusalem, Sch Comp Sci & Engn, IL-91904 Jerusalem, Israel
[3] Hebrew Univ Jerusalem, Dept Mol Genet & Biotechnol, Fac Med, IL-91120 Jerusalem, Israel
[4] Harvard Univ, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
来源：
SCIENCE | 2007年 / 315卷 / 5817期
关键词：
D O I：
10.1126/science.1134053
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Chromatin plays roles in processes governed by different time scales. To assay the dynamic behavior of chromatin in living cells, we used genomic tiling arrays to measure histone H3 turnover in G1-arrested Saccharomyces cerevisiae at single-nucleosome resolution over 4% of the genome, and at lower (similar to 265 base pair) resolution over the entire genome. We find that nucleosomes at promoters are replaced more rapidly than at coding regions and that replacement rates over coding regions correlate with polymerase density. In addition, rapid histone turnover is found at known chromatin boundary elements. These results suggest that rapid histone turnover serves to functionally separate chromatin domains and prevent spread of histone states.
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收藏
页码：1405 / 1408
页数：4
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