Genome-Wide Association Study on Resistance to Rice Black-Streaked Dwarf Disease Caused by Rice black-streaked dwarf virus

被引:13
作者
Liu, Qing [1 ]
Lan, Guofang [2 ,3 ]
Zhu, Yajun [4 ]
Chen, Kai [4 ]
Shen, Congcong [4 ]
Zhao, Xiuqin [1 ]
Zhang, Fan [1 ]
Xu, Jianlong [1 ]
Li, Zhikang [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
[2] Yangzhou Univ, Key Lab Plant Funct Genom, Minist Educ, Coll Biosci & Biotechnol, Yangzhou 225009, Jiangsu, Peoples R China
[3] Changshu Inst Agr Sci, Changshu 215500, Jiangsu, Peoples R China
[4] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr, Shenzhen 518120, Peoples R China
基金
中国国家自然科学基金;
关键词
genome-wide association study; haplotype analysis; Oryza sativa L; rice; rice black-streaked dwarf disease; Rice black-streaked dwarf virus; QUANTITATIVE TRAIT LOCI; SMALL BROWN PLANTHOPPER; AGRONOMIC TRAITS; IDENTIFICATION; SEQUENCE; MAIZE; MODEL; WHEAT; GWAS; QTLS;
D O I
10.1094/PDIS-10-19-2263-RE
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Rice black-streaked dwarf disease caused by Rice black-streaked dwarf virus (RBSDV) is one of the most destructive viral diseases of rice. Thus, it is imperative that resistant rice germplasms are screened for novel RBSDV-resistant genes. RBSDV resistance of a diverse global collection comprising 1,953 rice accessions was evaluated under natural conditions across 3 years. The average disease incidences of the Xian/indica (XI) subgroup were significantly lower than those of the Geng/japonica (GJ) subgroup. Interestingly, most XI-1A accessions in the Xian subgroup were significantly more susceptible than XI-1B accessions, even though XI-1A and XI-1B have a close phylogenetic relationship. Four Xian accessions stably and highly resistant to RBSDV were consistently identified in 2 years. Ten genomic regions (GRs) with 147 single nucleotide polymorphisms associated with RBSDV resistance were detected by a single-locus genome-wide association study (GWAS), of which five were repeatedly identified in a multilocus GWAS. Two previously reported GRs, grRBSDV-6.1 and grRBSDV-6.3, which were repeatedly detected as stably and highly associated with RBSDV resistance, contained 17 and seven genes, respectively, with significant differences of resistance among haplotypes. Haplotype analyses of the candidate genes LOC_Os06g03150 in grRBSDV-6.1 and LOC_Os06g31190 in grRBSDV-6.3 suggested that the former gene is mainly associated with the differentiation of resistance within the Xian subgroup and the latter gene mainly explains the difference in the resistance between Xian and Geng. Another three novel resistance GRs (grRBSDV-1.1, grRBSDV-7.1, and grRBSDV-9.1) were identified. Our findings may enhance the application of disease-resistant rice germplasms for breeding RBSDV-resistant varieties.
引用
收藏
页码:607 / 615
页数:9
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