Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry

被引：67
作者：
Neffling, Milla-Riina [1 ]
Lance, Emilie [2 ]
Meriluoto, Jussi [1 ]
机构：
[1] Abo Akad Univ, Dept Biochem & Pharm, FI-20520 Turku, Finland
[2] Univ Rennes 1, CNRS, UMR Ecobio 6553, F-35042 Rennes, France
来源：
ENVIRONMENTAL POLLUTION | 2010年 / 158卷 / 03期
关键词：
Microcystins; MMPB method; Total contamination levels; IN-VIVO EVIDENCE; PROTEIN PHOSPHATASES; LR; LIVER; CYANOBACTERIA; INHIBITOR; NODULARIN; TOXICITY; MUSSELS; BINDING;
D O I：
10.1016/j.envpol.2009.10.023
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Microcystins are cyanobacterial hepatotoxins capable of accumulation into animal tissues. The toxins act by inhibiting specific protein phosphatases and both non-covalent and covalent interactions occur. The 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) method determines the total, i e. the sum of free and protein-bound microcystin in tissues. The aim of the method development in this paper was to tackle the problems with the MMPB methodology. the rather laborious workflow and the loss of material during different steps of the method In the optimised workflow the oxidation recovery was of acceptable level (29-40%), the extraction efficiency good (62-97%), but the signal suppression effect from the matrix remained severe in our system (16-37% signal left) The extraction efficiency for the determination of the free, extractable microcystins, was found to be good, 52-100%, depending on the sample and the toxin variant and concentration (C) 2009 Elsevier Ltd. All rights reserved
引用
收藏
页码：948 / 952
页数：5
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