A comparison between the phenol-chloroform method of RNA extraction and the QIAamp viral RNA kit in the extraction of hepatitis C and GB virus-C/hepatitis G viral RNA from serum

被引：12

作者：

Fanson, BG ^{[1
]}

Osmack, P ^{[1
]}

Di Bisceglie, AM ^{[1
]}

机构：

[1] St Louis Univ, Sch Med, Dept Internal Med, St Louis, MO 63104 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 2000年 / 89卷 / 1-2期

关键词：

hepatitis C virus; GBV-C/HGV; QIAamp viral RNA; sensitivity; RNA;

D O I：

10.1016/S0166-0934(00)00192-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Viral RNAs are detected commonly in serum by hybridization and polymerase chain reaction (PCR) methods for both clinical and research purposes. Two methods of extracting Viral RNA were evaluated prior to amplification by PCR. One was a conventional phenol-chloroform method and the other used a standardized, manufactured kit. The efficiency of extraction was tested by semi-quantitative amplification of hepatitis C viral and GB virus-C/hepatitis G viral RNAs. The standarized commercial method, although more time efficient, resulted in an approximately ten fold less sensitivity. Thus, in situations where maximum sensitivity is needed, the more labor intenstive phenol choloroform method is recommended. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：23 / 27

页数：5

共 15 条

[11] Molecular cloning and disease association of hepatitis G virus: A transfusion-transmissible agent [J].

Linnen, J ;

Wages, J ;

ZhangKeck, ZY ;

Fry, KE ;

Krawczynski, KZ ;

Alter, H ;

Koonin, E ;

Gallagher, M ;

Alter, M ;

Hadziyannis, S ;

Karayiannis, P ;

Fung, K ;

Nakatsuji, Y ;

Shih, JWK ;

Young, L ;

Piatak, M ;

Hoover, C ;

Fernandez, J ;

Chen, S ;

Zou, JC ;

Morris, T ;

Hyams, KC ;

Ismay, S ;

Lifson, JD ;

Hess, G ;

Foung, SKH ;

Thomas, H ;

Bradley, D ;

Margolis, H ;

Kim, JP .

SCIENCE, 1996, 271 (5248) :505-508

[12] Interferon alfa-2b alone or in combination with ribavirin as initial treatment for chronic hepatitis C [J].

McHutchison, JG ;

Gordon, SC ;

Schiff, ER ;

Shiffman, ML ;

Lee, WM ;

Rustgi, VK ;

Goodman, ZD ;

Ling, MH ;

Cort, S ;

Albrecht, JK .

NEW ENGLAND JOURNAL OF MEDICINE, 1998, 339 (21) :1485-1492

[13] Comparison of QIAamp HCV kit spin columns, silica beads, and phenol-chloroform for recovering human immunodeficiency virus type 1 RNA from plasma [J].

Shafer, RW ;

Levee, DJ ;

Winters, MA ;

Richmond, KL ;

Huang, D ;

Merigan, TC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (02) :520-522

[14] VOLUNTEER BLOOD-DONORS WITH ANTIBODY TO HEPATITIS-C VIRUS - CLINICAL, BIOCHEMICAL, VIROLOGICAL, AND HISTOLOGIC FEATURES [J].

SHAKIL, AO ;

CONRYCANTILENA, C ;

ALTER, HJ ;

HAYASHI, P ;

KLEINER, DE ;

TEDESCHI, V ;

KRAWCZYNSKI, K ;

CONJEEVARAM, HS ;

SALLIE, R ;

DIBISCEGLIE, AM ;

MELPOLDER, JC ;

HOOFNAGLE, JH .

ANNALS OF INTERNAL MEDICINE, 1995, 123 (05) :330-337

[15] DECREASE IN SERUM HEPATITIS-C VIRAL-RNA DURING ALPHA-INTERFERON THERAPY FOR CHRONIC HEPATITIS-C [J].

SHINDO, M ;

DIBISCEGLIE, AM ;

CHEUNG, L ;

SHIH, JWK ;

CRISTIANO, K ;

FEINSTONE, SM ;

HOOFNAGLE, JH .

ANNALS OF INTERNAL MEDICINE, 1991, 115 (09) :700-704

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