Microbial communities involved in Kasar cheese ripening

被引:19
作者
Aydemir, Oguz [1 ]
Harth, Henning [2 ]
Weckx, Stefan [2 ]
Dervisoglu, Muhammet [3 ]
De Vuyst, Luc [2 ]
机构
[1] Cankiri Karatekin Univ, Fac Engn, Dept Food Engn, TR-18100 Cankiri, Turkey
[2] Vrije Univ Brussel, Fac Sci & Bioengn Sci, Dept Bioengn Sci, Res Grp Ind Microbiol & Food Biotechnol IMDO, B-1050 Brussels, Belgium
[3] Ondokuz Mayis Univ, Fac Engn, Dept Food Engn, TR-55139 Samsun, Turkey
关键词
Kasar cheese; Cheese ripening; Non-starter lactic acid bacteria; LACTIC-ACID BACTERIA; SENSORY CHARACTERISTICS; ITALIAN CHEESE; KASHAR CHEESE; MILK; MANUFACTURE; IDENTIFICATION; ENTEROCOCCI; MICROFLORA; FOOD;
D O I
10.1016/j.fm.2014.10.002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The microbiota of non-starter lactic acid bacteria (NSLAB) and their concomitant community dynamics during cheese ripening were investigated for traditional Turkish Kasar cheeses made from raw cows' milk. Five batches of 15 Kasar cheeses produced in different dairy plants located in Kars wereanalysed during their whole ripening phase up to 180 days. Lactobacilli and lactococci were determined as the prevailing microbial groups. The molecular classification and identification of 594 LAB isolates during Kasar cheese ripening were performed through (GTG)(5)-PCR fingerprinting of their genomic DNA followed by verification of the (GTG)(5)-PCR clusters obtained after numerical analysis through 16S rRNA gene sequencing of representative isolates. Lactobacillus casei (247 isolates, 41.6%), Lactobacillus plantarum (77 isolates, 13.0%), and Pediococcus acidilactici (58 isolates, 9.8%) were the prevailing NSLAB species in all Kasar cheeses of the different dairy plants investigated throughout cheese ripening. The data of the present study contribute to the inventory of unique cheese varieties to enable the prevention of losses of microbial biodiversity and the selection of starter cultures for controlled cheese manufacturing. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:587 / 595
页数:9
相关论文
共 55 条
[1]   Lactic acid bacteria occurring during manufacture and ripening of Provolone del Monaco cheese: Detection by different analytical approaches [J].
Aponte, Maria ;
Fusco, Vincenzina ;
Andolfi, Rosamaria ;
Coppola, Salvatore .
INTERNATIONAL DAIRY JOURNAL, 2008, 18 (04) :403-413
[2]  
Aran N, 1998, MILCHWISSENSCHAFT, V53, P565
[3]  
Beresford T., 2004, CHEESE CHEM PHYS MIC, V1, P287, DOI [10.1016/S1874-558X(04)80071-X, DOI 10.1016/S1874-558X(04)80071-X]
[4]   Recent advances in cheese microbiology [J].
Beresford, TP ;
Fitzsimons, NA ;
Brennan, NL ;
Cogan, TM .
INTERNATIONAL DAIRY JOURNAL, 2001, 11 (4-7) :259-274
[5]   Unraveling the microbiota of teat apices of clinically healthy lactating dairy cows, with special emphasis on coagulase-negative staphylococci [J].
Braem, G. ;
De Vliegher, S. ;
Verbist, B. ;
Piessens, V. ;
Van Coillie, E. ;
De Vuyst, L. ;
Leroy, F. .
JOURNAL OF DAIRY SCIENCE, 2013, 96 (03) :1499-1510
[6]  
Çetinkaya F, 2006, TURK J VET ANIM SCI, V30, P397
[7]   Interactions between Lactococcus lactis and Streptococcus thermophilus strains in Cheddar cheese processing conditions [J].
Champagne, C. P. ;
Gagnon, D. ;
St-Gelais, D. ;
Vuillemard, J. C. .
INTERNATIONAL DAIRY JOURNAL, 2009, 19 (11) :669-674
[8]  
Cibik R, 2010, REV MED VET-TOULOUSE, V161, P509
[9]   Survey of lactic acid bacteria during the ripening of Caciocavallo cheese produced in Molise [J].
Coppola, R ;
Succi, M ;
Sorrentino, E ;
Iorizzo, M ;
Grazia, L .
LAIT, 2003, 83 (03) :211-222
[10]   Molecular evaluation of microbial diversity occurring in different types of Mozzarella cheese [J].
Coppola, S ;
Blaiotta, G ;
Ercolini, D ;
Moschetti, G .
JOURNAL OF APPLIED MICROBIOLOGY, 2001, 90 (03) :414-420