Injection molded chips with integrated conducting polymer electrodes for electroporation of cells

被引:36
作者
Andresen, Kristian Odegaard [1 ]
Hansen, Morten [1 ]
Matschuk, Maria [1 ]
Jepsen, Soren Terpager [2 ]
Sorensen, Henrik Schiott [2 ]
Utko, Pawel [1 ]
Selmeczi, David [1 ]
Hansen, Thomas S. [1 ]
Larsen, Niels B. [1 ]
Rozlosnik, Noemi [1 ]
Taboryski, Rafael [1 ]
机构
[1] Tech Univ Denmark, Dept Micro & Nanotechnol, DTU Nanotech, DK-4000 Roskilde, Denmark
[2] Tech Univ Denmark, Dept Photon Engn, DTU Foton, DK-4000 Roskilde, Denmark
关键词
MICROFLUIDIC DEVICES; SMALL MOLECULES; CHANNEL; SINGLE; IMPEDANCE; DELIVERY; SYSTEMS; DNA;
D O I
10.1088/0960-1317/20/5/055010
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented. The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz, +/- 50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.
引用
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页数:9
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