Protective effect of Letinous edodes foot peptides against ethanol-induced liver injury in L02 cells

被引：12
作者：
Ma, Lin [1 ,2 ]
Huo, Chun-Yan [1 ]
Zhang, Xiao-Yu [1 ]
Qin, Chen-Qiang [1 ]
Ren, Di-Feng [1 ]
Lu, Jun [2 ]
机构：
[1] Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing Key Lab Forest Food Proc & Safety, 35 Qinghua East Rd, Beijing 100083, Peoples R China
[2] China Natl Res Inst Food & Fermentat Ind, Beijing Engn Res Ctr Prot & Funct Peptides, Bldg 6,Courtyard 24,Jiuxianqiao Middle Rd, Beijing 100015, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2018年 / 18卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Letinous edodes foot peptide; ethanol-induced liver injury; antioxidation; anti- inflammatory effect; L02; cell; LENTINUS-EDODES; ANTIOXIDANT PROPERTIES; SCHISANDRA-CHINENSIS; OXIDATIVE INJURY; EDIBLE MUSHROOMS; FRUITING BODIES; GENE-EXPRESSION; HEPG2; CELLS; IN-VITRO; MICE;
D O I：
10.3892/mmr.2018.9093
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The aim of the present study was to evaluate the protective effect and mechanism of Letinous edodes foot peptides on ethanol-induced L02 cells. A cell model of ethanol-induced damage was established in vitro to study the effects of the Letinous edodes foot peptides on human L02 hepatocytes. The expression and activity of superoxide dismutase (SOD), malondialdehyde (MDA), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), following treatment were examined to determine the anti-alcoholism and hepatoprotective functions of Letinous edodes foot peptides. Taking Letinous edodes foot peptides prior to ethanol exposure was more beneficial, which significantly increased SOD activity and the mRNA expression of ADH and ALDH suppressed by ethanol. In addition, the intracellular MDA content, and AST and ALT activity decreased in ethanol-induced L02 cells pretreated with the peptides, when compared with the control. Furthermore, Letinous edodes foot peptides inhibited the ethanol-induced activation of the proinflammatory cytokines, interleukin-6 and tumor necrosis factor-, and promoted the metabolic regulation factors, AMP-activated protein kinase-2 and peroxisome proliferator-activated receptor-.
引用
收藏
页码：1858 / 1866
页数：9
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