Rotenoids from Clitoria fairchildiana R. Howard (Fabaceae) seeds affect the cellular metabolism of larvae of Aedes aegypti L. (Culicidae)

被引：1

作者：

Bertonceli, Maria Aparecida Aride ^{[1
]}

Oliveira, Antonia Elenir Amancio ^{[1
]}

Passos, Michel de Souza ^{[2
]}

Vieira, Ivo Jose Curcino ^{[2
]}

Braz-Filho, Raimundo ^{[2
]}

Lemos, Francisco Jose Alves ^{[3
]}

Martins, Brunna Xavier ^{[4
]}

Facanh, Arnoldo Rocha ^{[4
]}

Pireda, Saulo ^{[4
]}

da Cunha, Maura ^{[4
]}

Fernandes, Katia Valevski Sales ^{[1
]}

机构：

[1] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Quim & Funcao Prot & Peptideos, Ave Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ, Brazil

[2] Univ Estadual Norte Fluminense, Ctr Ciencia & Tecnol, Lab Ciencias Quim, Ave Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ, Brazil

[3] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Biotecnol, Ave Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ, Brazil

[4] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Biol Celular & Tecidual, Ave Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ, Brazil

来源：

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | 2022年 / 186卷

关键词：

Glucopyranosylrotenoid; Butterfly pea tree; Dengue mosquito; Oxidative stress; VH+-ATPase activity; MIDGUT; RESISTANCE; STRESS; ATPASE; DENGUE; DEATH;

D O I：

10.1016/j.pestbp.2022.105167

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Non-domesticated species may represent a treasure chest of defensive molecules which must be investigated and rescued. Clitoria fairchildiana R. Howard is a non-domesticated Fabacea, native from the Amazonian Forest whose seeds are exquisitely refractory to insect predation. Secondary metabolites from these seeds were fractionated by different organic solvents and the CH2Cl2 fraction (CFD - Clitoria fairchildiana dichloromethane fraction), as the most toxic to 3rd instar Aedes aegypti larvae (LC50 180 PPM), was subjected to silica gel chromatography, eluted with a gradient of CH2Cl2: MeOH and sub fractioned in nine fractions (CFD1 -CFD9). All obtained fractions were tested in their toxicity to the insect larvae. Two rotenoids, a 11 alpha-O-beta-D-glucopyranosylrotenoid and a 6-deoxy-clitoriacetal 11-O-n-glucopyranoside, were identified in the mixture of CFD 7.4 and CFD 7.5, and they were toxic (LC(50 )120 PPM) to 3rd instar Ae. aegypti larvae, leading to exoskeleton changes, cuticular detachment and perforations in larval thorax and abdomen. These C. fairchildiana rotenoids interfered with the acidification process of cell vesicles in larvae midgut and caused inhibition of 55% of V-ATPases activity of larvae treated with 80 PPM of the compounds, when compared to control larvae. The rotenoids also led to a significant increase in the production of reactive oxygen species (ROS) in treated larvae, especially in the hindgut region of larvae intestines, indicating a triggering of an oxidative stress process to these insects.

引用

页数：7

共 29 条

[1] Exposure to carbaryl leads to ultrastructural changes and alters activity of antioxidant enzymes in Spodoptera exigua (Lepidoptera: Noctuidae) [J].