Sandwich-type amperometric immunosensor for cancer biomarker based on signal amplification strategy of multiple enzyme-linked antibodies as probes modified with carbon nanotubes and concanavalin A

被引：27
作者：
Yang, Penghao [1 ]
Li, Xueqin [1 ]
Wang, Lisha [1 ]
Wu, Qi [1 ]
Chen, Zhichun [1 ]
Lin, Xianfu [1 ]
机构：
[1] Zhejiang Univ, Dept Chem, Hangzhou 310027, Zhejiang, Peoples R China
来源：
JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2014年 / 732卷
基金：
中国国家自然科学基金;
关键词：
Carcinoembryonic antigen; Multiwalled carbon nanotubes; Concanavalin A; Cysteine; ULTRASENSITIVE ELECTROCHEMICAL IMMUNOSENSOR; CARCINOEMBRYONIC ANTIGEN; GOLD NANOPARTICLES; HORSERADISH-PEROXIDASE; MULTILAYER FILMS; IMMUNOASSAY; CEA; BIENZYME; IMMOBILIZATION; NANOSPHERES;
D O I：
10.1016/j.jelechem.2014.08.030
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A sandwich-type amperometric immunosensor for carcinoembryonic antigen was fabricated by using functionalized carbon nanotubes decorated with concanavalin A along with horseradish peroxidase-labeled carcinoembryonic secondary antibodies. The HRP-Ab(2) was assembled onto carbon nanotubes templates based on the biospecific affinity between concanavalin A and HRP. The carcinoembryonic antibody was immobilized on the gold electrode modified by monolayer cysteine. The response is linear in the range of 0.05-5 ng mL(-1) and 5-200 ng mL(-1), with a detection limit of 0.018 ng mL(-1). The biosensor can be used in serum directly. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：38 / 45
页数：8
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