Extended target sequence specificity of PNA-minor-groove binder conjugates

被引：7
|
作者：
Nielsen, PE [1 ]
Frederiksen, K [1 ]
Behrens, C [1 ]
机构：
[1] Univ Copenhagen, Panum Inst, Dept Med Biochem & Genet, DK-2200 Copenhagen, Denmark
来源：
CHEMBIOCHEM | 2005年 / 6卷 / 01期
关键词：
DNA recognition; DNA targeting; minor-groove binder; nucleic acids; peptide nucleic acids;
D O I：
10.1002/cbic.200400251
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Conjugal bliss. We show that a peptide nucleic acid-Hoechst conjugate (see scheme) kinetically and sequence preferentially guides the PNA moiety to target a binding site proximal to an A-T region that has an affinity for the minor-groove binder. These results demonstrate a new strategy for constructing DNA recognition ligands composed of a sequence-guiding domain that increases the target specificity and a DNA-modification domain that determines the biological activity of the conjugate.
引用
收藏
页码：66 / 68
页数：3
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