Optimal conditions for enhanced β-mannanase production by recombinant Aspergillus sojae

被引：31

作者：

Ozturk, Bengu ^{[1
]}

Cekmecelioglu, Deniz ^{[1
]}

Ogel, Zumrut Begum ^{[1
]}

机构：

[1] Middle E Tech Univ, Dept Food Engn, METU, TR-06531 Ankara, Turkey

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2010年 / 64卷 / 3-4期

关键词：

Recombinant Aspergillus sojae; Endo-beta-1,4-mannanase; Response surface methodology; Box-Behnken; ALPHA-AMYLASE; OPTIMIZATION; PURIFICATION; ENZYMES; NIGER; ENDO-BETA-1,4-MANNANASE; HEMICELLULOSE; FERMENTATION; HYDROLYSIS; EXPRESSION;

D O I：

10.1016/j.molcatb.2010.02.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Optimization of the growth conditions for maximum P-mannanase production in shake flasks by using recombinant Aspergillus sojae ATCC11906 (AsT1) was carried out by Box-Behnken design of response surface methodology. The highest beta-mannanase activity on the fourth day of cultivation at 30 degrees C was obtained as 363 U/ml in the optimized medium consisting of 7% sugar beet molasses, 0.43% NH(4)NO(3), 0.1% K(2)HPO(4) and 0.05% MgSO(4) (by weight per volume) at 207 rpm. On the sixth day of cultivation under the optimized conditions, the highest beta-mannanase activity was achieved as 482 U/ml which is 1.4-fold of 352 U/ml activity found on glucose medium previously. (C) 2010 Elsevier BM. All rights reserved.

引用

页码：135 / 139

页数：5

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