Testosterone and FSH modulate Sertoli cell extracellular secretion: Proteomic analysis

被引：28

作者：

Mancuso, Francesca ^{[1
]}

Calvitti, Mario ^{[1
]}

Milardi, Domenico ^{[2
,3
]}

Grande, Giuseppe ^{[2
]}

Falabella, Giulia ^{[1
]}

Arato, Iva ^{[1
]}

Giovagnoli, Stefano ^{[4
]}

Vincenzoni, Federica ^{[5
]}

Mancini, Francesca ^{[2
]}

Nastruzzi, Claudio ^{[6
]}

Bodo, Maria ^{[1
]}

Baroni, Tiziano ^{[1
]}

Castagnola, Massimo ^{[5
]}

Marana, Riccardo ^{[2
]}

Pontecorvi, Alfredo ^{[2
,3
]}

Calafiore, Riccardo ^{[7
,8
,9
]}

Luca, Giovanni ^{[1
,7
,8
]}

机构：

[1] Univ Perugia, Dept Expt Med, I-06100 Perugia, Italy

[2] Int Sci Inst Paul VI, I-00168 Rome, Italy

[3] Fdn Policlin A Gemelli, Div Endocrinol, I-00168 Rome, Italy

[4] Univ Perugia, Dept Pharmaceut Sci, I-06100 Perugia, Italy

[5] Catholic Univ, Inst Chem & Clin Biochem, I-00168 Rome, Italy

[6] Univ Ferrara, Dept Life Sci & Biotechnol, I-44121 Ferrara, Italy

[7] Univ Perugia, Div Med Androl & Endocrinol Reprod, I-05100 Terni, Italy

[8] St Mary Hosp, I-05100 Terni, Italy

[9] Univ Perugia, Dept Med, I-06100 Perugia, Italy

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2018年 / 476卷

关键词：

Sertoli cells; Extracellular vesicles; Proteomic analysis; mRNAs; FOLLICLE-STIMULATING-HORMONE; SINGLE NUCLEOTIDE POLYMORPHISM; RAT SEMINIFEROUS EPITHELIUM; JUNCTION DYNAMICS; IN-VIVO; PHARMACOGENETIC APPROACH; ADHERING JUNCTIONS; MALE-INFERTILITY; RECEPTOR GENE; INHIBIN-B;

D O I：

10.1016/j.mce.2018.04.001

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Spermatogenesis is a highly complicated biological process that occurs in the epithelium of the seminiferous tubules. It is regulated by a complex network of endocrine and paracrine factors and by juxtacrine testicular cross-talk. Sertoli cells (SC) play a key role in spermatogenesis due to their production of trophic, differentiation and immune-modulating factors, but many of the molecular pathways of SC action remain controversial and unclear. Over the last two decades, research has focused on extracellular vesicles as an important mechanism of intercellular communication. The aim of this study was to investigate the presence of extracellular vesicles (EVs) in SC and the modulation of their content in SC after FSH and testosterone stimulation. Highly purified porcine pre-pubertal Sertoli cells were isolated according to previously established methods. After 48 h of culture with FSH or FSH + testosterone stimulation, we identified sertolian EVs containing specific mRNAs. Proteomic analysis of EVs content identified 29 proteins under non-stimulatory conditions, most of which were related to receptor binding activity. FSH stimulation induced increases in inhibin-alpha, inhibin-beta, plakoglobin, haptoglobin, D-3-phosphoglycerate dehydrogenase and sodium/potassium-transporting ATPase in sertolian EVs. Testosterone stimulation enhanced the abundance of inhibin-alpha, inhibin-beta, tissue-type plasminogen activator, epidermal growth factor-like protein 8, elongating factor 1-gamma and D-3-phosphoglycerate dehydrogenase. These results are likely to help determine the unknown molecular secretion of Sertoli cells.

引用

页码：1 / 7

页数：7

共 48 条

[1] MUTATION IN THE FOLLICLE-STIMULATING-HORMONE RECEPTOR GENE CAUSES HEREDITARY HYPERGONADOTROPIC OVARIAN FAILURE [J].