Anatomical and molecular studies of cytochrome P450 family CYP7A1 gene polymorphism and its association with gallstone in north Indian population

Introduction: In this study, we have mainly focused on the molecular and biochemical aspects to know the cause of gall stone diseases in human. To know about these problems, there may be necessitating initiating interest in researchers of this field and to set up easily available tools and techniques. The screening of polymorphism with molecular approaches of P450 super family CYP7A1 gene was performed to explore its relation with gallstone diseases. Methods: Total 300 samples (150 patients and 150 controls) were analyzed for the study. The polymorphisms were analyzed by PCR followed by RFLP with Bsal restriction enzyme. The lipid profile was estimated by using modified Roeschlau's method. The cholesterol content in gall stones was determined with Liebermann-Burchard reaction method. Results: The cholesterol content of recovered gallstones was found 98.86 +/- 25.43 (% by weight). BMI and serum glucose were found higher in patients than in the control group: 30.84 +/- 8.13 kg/m(2) vs 28.61 +/- 7.50 kg/m(2) (P = 0.032), and 121.03 +/- 15.11 mg/dL vs 104 +/- 21.05 mg/dL (P = 0.001) respectively. Statistical analysis showed no significant differences in genotypic frequencies of gene CYP7A1 gene polymorphism between patients and controls. Frequencies of C allele (CYP7A1 gene polymorphism) in patients and controls were obtained 24.24% vs 26.46% (P = 0.621, OR = 0.89). Genotypic frequencies between patients and controls were found 59.42% vs 54.97% for AA; 30.25% vs 34.72% for CA; and 10.33% vs 10.31 for CC. Discussion: In this study by using of multiple logistic regression analysis the results have indicated that CYP7A1 gene polymorphism may not play any significant role in gallstone disease. (C) 2018 Anatomical Society of India. Published by Elsevier, a division of RELX India, Pvt. Ltd. All rights reserved.