Peroxisomal proteomics: Biomonitoring in mussels after the Prestige's oil spill

被引：33

作者：

Apraiz, Itxaso ^{[1
]}

Cajaraville, Miren P. ^{[2
]}

Cristobal, Susana ^{[1
]}

机构：

[1] Stockholm Univ, Arrhenius Labs Nat Sci, Dept Biochem & Biophys, SE-10691 Stockholm, Sweden

[2] Univ Basque Country, Fac Sci & Technol, Dept Zool & Cell Biol, E-48080 Bilbao, Basque Country, Spain

来源：

MARINE POLLUTION BULLETIN | 2009年 / 58卷 / 12期

基金：

瑞典研究理事会;

关键词：

Proteomics; Biomarker; Two-dimensional electrophoresis; Marine pollution assessment; Oil spill; Prestige; MYTILUS-GALLOPROVINCIALIS LMK; RAT-LIVER; 2-DIMENSIONAL ELECTROPHORESIS; DIGESTIVE GLAND; CRUDE-OIL; PROTEINS; EDULIS; EXPRESSION; POLLUTANTS; POLLUTION;

D O I：

10.1016/j.marpolbul.2009.08.004

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Peroxisomal proteomics was applied to assess possible biological effects after the Prestige's oil spill. Mussels were sampled in July 2004 and 2005 in four stations in the NW (closest to the spill) and NE coasts of the Iberian Peninsula. Principal components analysis (PCA) suggested differences in protein expression among stations and sampling years. Several proteins were putatively identified by mass spectrometry and immunolocalization. PCI separated the NW stations in 2004 from the rest of the stations and sampling years mainly due to up-regulation of peroxisomal P-oxidation proteins and PMP70. PC3 separated the NE stations, based on up-regulation of the antioxidant enzyme catalase in 2004 compared to 2005. PC4 separated the stations in the NE and the NW. This work shows that environmental proteomics, together with multivariate data analysis, could provide information to interpret the effects of oil spills at cellular level in mussels. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1815 / 1826

页数：12

共 51 条

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