Preparation of a polybutadiene stationary phase immobilized by γ radiation for reversed-phase high-performance liquid chromatography

被引：14

作者：

Lopes, NP ^{[1
]}

Collins, KE ^{[1
]}

Jardim, ICSF ^{[1
]}

机构：

[1] Univ Estadual Campinas, Inst Quim, Lab Pesquisas Cromatog Liquida, LABCROM, BR-13084971 Campinas, SP, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 987卷 / 1-2期

关键词：

stationary phases; LC; polybutadiene-silica stationary phases;

D O I：

10.1016/S0021-9673(02)01658-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polybutadiene (PBD) has been immobilized on HPLC silica by gamma radiation doses in the range from 5 to 180 kGy. Columns prepared from these reversed-phase materials, as well as from similar non-irradiated materials, were tested with standard sample mixtures and characterized by elemental analysis (% C) and infrared spectroscopy. A low dose of 5 kGy is sufficient to produce a layer of immobilized PBD which functions as an efficient and stable stationary phase. Higher doses give thicker immobilized layers having less favorable chromatographic properties. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：77 / 85

页数：9

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