Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants

被引：543

作者：

Elshikh, Mohamed ^{[1
]}

Ahmed, Syed ^{[1
]}

Funston, Scott ^{[1
]}

Dunlop, Paul ^{[1
]}

McGaw, Mark ^{[1
]}

Marchant, Roger ^{[1
]}

Banat, Ibrahim M. ^{[1
]}

机构：

[1] Univ Ulster, Sch Biomed Sci, Pharmaceut Sci & Practice Res Grp, Cromore Rd, Coleraine BT52 1SA, Londonderry, North Ireland

来源：

BIOTECHNOLOGY LETTERS | 2016年 / 38卷 / 06期

关键词：

Antibiotics; Biosurfactant; Minimum inhibitory concentration; Resazurin; DILUTION METHODS; AGAR;

D O I：

10.1007/s10529-016-2079-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To develop and validate a microdilution method for measuring the minimum inhibitory concentration (MIC) of biosurfactants. A standardized microdilution method including resazurin dye has been developed for measuring the MIC of biosurfactants and its validity was established through the replication of tetracycline and gentamicin MIC determination with standard bacterial strains. This new method allows the generation of accurate MIC measurements, whilst overcoming critical issues related to colour and solubility which may interfere with growth measurements for many types of biosurfactant extracts.

引用

页码：1015 / 1019

页数：5

共 13 条

[1] Comparison of Broth Microdilution, Agar Dilution, and Etest for Susceptibility Testing of Doripenem against Gram-Negative and Gram-Positive Pathogens [J].