An automated and portable microfluidic chemiluminescence immunoassay for quantitative detection of biomarkers

被引：100

作者：

Hu, Binfeng ^{[1
,2
]}

Li, Juanjuan ^{[1
]}

Mou, Lei ^{[1
,2
]}

Liu, Yong ^{[1
,2
]}

Deng, Jinqi ^{[1
,2
]}

Qian, Wei ^{[1
,2
]}

Sun, Jiashu ^{[1
,2
]}

Cha, Ruitao ^{[1
]}

Jiang, Xingyu ^{[1
,2
]}

机构：

[1] Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

LAB ON A CHIP | 2017年 / 17卷 / 13期

关键词：

C-REACTIVE PROTEIN; CIRCULATING TUMOR-CELLS; CARE; PLATFORM; SYSTEM; CHIPS; AMPLIFICATION; MULTIPLEX; LAB; PATHOGENS;

D O I：

10.1039/c7lc00249a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microfluidic platforms capable of automated, rapid, sensitive, and quantitative detection of biomarkers from patient samples could make a major impact on clinical or point-of-care (POC) diagnosis. In this work, we realize an automated diagnostic platform composed of two main components: (1) a disposable, self-contained, and integrated microfluidic chip and (2) a portable instrument that carries out completely automated operations. To demonstrate its potential for real-world application, we use injection molding for mass fabrication of the main components of disposable microfluidic chips. The assembled three-layered chip with on-chip mechanical valves for fluid control consists of (1) a top silicone fluidic layer with embedded zigzag microchannels, reagent reservoirs and a negative pressure port, (2) a middle tinfoil layer with patterned antibody/antigen stripes, and (3) a bottom silicone substrate layer with waste reservoirs. The versatility of the microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of C-reactive protein (CRP) and testosterone in real clinical samples. This lab-on-a-chip platform with features of quantitation, portability and automation provides a promising strategy for POC diagnosis.

引用

页码：2225 / 2234

页数：10

共 58 条

[1] C-reactive protein [J].