Sample preparation in foodomic analyses

被引:15
作者
Martinovic, Tamara [1 ]
Gajdosik, Martina Srajer [2 ]
Josic, Djuro [1 ,3 ]
机构
[1] Univ Rijeka, Dept Biotechnol, Rijeka, Croatia
[2] JJ Strossmayer Univ Osijek, Dept Chem, Osijek, Croatia
[3] Brown Univ, Brown Med Sch, Dept Med, One Hoppin St, Providence, RI 02903 USA
关键词
Genomics; Lipidomics; Metabolomics; Proteomics; Sample preparation; PERFORMANCE LIQUID-CHROMATOGRAPHY; PRESSURE CHEMICAL-IONIZATION; TANDEM MASS-SPECTROMETRY; MALDI-TOF MS; QUADRUPOLE-TIME; GC-MS; QUANTITATIVE PROTEOMICS; METABOLOMIC ANALYSIS; MONOLITHIC SUPPORTS; SHOTGUN LIPIDOMICS;
D O I
10.1002/elps.201800029
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Representative sampling and adequate sample preparation are key factors for successful performance of further steps in foodomic analyses, as well as for correct data interpretation. Incorrect sampling and improper sample preparation can be sources of severe bias in foodomic analyses. It is well known that both wrong sampling and sample treatment cannot be corrected anymore. These, in the past frequently neglected facts, are now taken into consideration, and the progress in sampling and sample preparation in foodomics is reviewed here. We report the use of highly sophisticated instruments for both high-performance and high-throughput analyses, as well as miniaturization and the use of laboratory robotics in metabolomics, proteomics, peptidomics, and genomics.
引用
收藏
页码:1527 / 1542
页数:16
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