EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells

被引:129
作者
Galler, K. M. [1 ]
Widbiller, M. [1 ]
Buchalla, W. [1 ]
Eidt, A. [1 ]
Hiller, K. -A. [1 ]
Hoffer, P. C. [1 ]
Schmalz, G. [1 ,2 ]
机构
[1] Univ Med Ctr, Dept Conservat Dent & Periodontol, Franz Josef Str Allee 11, D-93053 Regensburg, Germany
[2] Univ Bern, Sch Dent Med, ZMK Bern, Bern, Switzerland
关键词
dental pulp stem cells; dentine; EDTA; growth factors; regenerative endodontics; MATRIX COMPONENTS; GROWTH-FACTORS; TGF-BETA; IGF-I; SOLUBILIZATION; ENDODONTICS; RELEASE; BINDING; FGF2;
D O I
10.1111/iej.12492
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Aim To evaluate the effect of dentine conditioning on migration, adhesion and differentiation of dental pulp stem cells. Methodology Dentine discs prepared from extracted human molars were pre-treated with EDTA (10%), NaOCl (5.25%) or H-2 O. Migration of dental pulp stem cells towards pre-treated dentine after 24 and 48 h was assessed in a modified Boyden chamber assay. Cell adhesion was evaluated indirectly by measuring cell viability. Expression of mineralization-associated genes (COL1A1, ALP, BSP, DSPP, RUNX2) in cells cultured on pre-treated dentine for 7 days was determined by RT-qPCR. Nonparametric statistical analysis was performed for cell migration and cell viability data to compare different groups and time-points (Mann-Whitney U-test, alpha = 0.05). Results Treatment of dentine with H2O or EDTA allowed for cell attachment, which was prohibited by NaOCl with statistical significance (P = 0.000). Furthermore, EDTA conditioning induced cell migration towards dentine. The expression of mineralization-associated genes was increased in dental pulp cells cultured on dentine after EDTA conditioning compared to H2O-pre-treated dentine discs. Conclusions EDTA conditioning of dentine promoted the adhesion, migration and differentiation of dental pulp stem cells towards or onto dentine. A pretreatment with EDTA as the final step of an irrigation protocol for regenerative endodontic procedures has the potential to act favourably on new tissue formation within the root canal.
引用
收藏
页码:581 / 590
页数:10
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